Description

Lyticase is a highly purified enzyme complex derived from *Arthrobacter luteus*, widely recognized for its ability to degrade yeast cell walls. This product is essential for the efficient extraction of intracellular components from yeast and other fungi, a critical step in numerous biotechnological and research workflows. It is a vital reagent for professionals in the biotechnology, pharmaceutical, and life science research sectors, enabling applications from protein production to genomic DNA isolation.

Application

• Yeast cell wall lysis for the extraction of recombinant proteins, enzymes, and other intracellular products.
• Preparation of yeast spheroplasts or protoplasts for genetic transformation, fusion, and other cellular biology studies.
• Isolation of high-quality genomic DNA, RNA, or plasmids from yeast and fungal species.
• Essential component in protocols for studying yeast cell wall composition and structure.
• Used in the food and beverage industry for yeast autolysis processes.
• Facilitates the release of periplasmic proteins from yeast expression systems.

Basic Information

Product Name	Lyticase
CAS No.	144941-36-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	β-1,3-Glucanase; Zymolyase; Yeast Cell Wall Lytic Enzyme; Arthrobacter Luteus Lytic Enzyme; 1,3-β-Glucan 3-Glucanohydrolase; Glucan Endo-1,3-β-D-Glucosidase; Endo-1,3(4)-β-Glucanase
EINECS	Contact for details

Quality Control

Our Lyticase is produced under strict quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is tested for specific activity, protein content, and absence of contaminating nucleases and proteases. A comprehensive Certificate of Analysis (COA) detailing purity, activity units, and storage stability is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For extended use, aliquot the product to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms
Protein Content (Biuret)	≥ 90%
Specific Activity	≥ 20,000 units/mg protein
pH (in solution)	6.0 - 7.5
Endotoxin (LAL)	< 1.0 EU/mg
DNase & RNase Activity	Not detected
Protease Activity	Not detected

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.