Description

Rpd3 Protein CAS NO 144714-44-3 is a highly purified recombinant protein, specifically a histone deacetylase (HDAC) enzyme, crucial for epigenetic research and therapeutic development. This protein is essential for studying chromatin remodeling, gene expression regulation, and identifying novel inhibitors for cancer and neurological disorders. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and biotechnology companies focused on epigenetics and drug discovery.

Application

• Biochemical Research: Used as a key enzyme in in vitro assays to study histone deacetylation mechanisms and chromatin dynamics.
• Drug Discovery & Screening: Serves as a primary target for high-throughput screening (HTS) to identify and validate novel HDAC inhibitors for therapeutic applications.
• Enzyme Kinetics Studies: Employed to determine kinetic parameters (Km, Vmax) and characterize the inhibitory effects of potential drug candidates.
• Protein-Protein Interaction Analysis: Used in pull-down assays, co-immunoprecipitation (Co-IP), and other techniques to map interaction partners within transcriptional complexes.
• Antibody Validation & Specificity Testing: Acts as a positive control and antigen for validating the specificity of antibodies targeting HDAC family proteins.
• Educational & Diagnostic Kit Development: Incorporated into research kits and diagnostic assays for teaching and commercial purposes related to epigenetic pathways.

Basic Information

Product Name	Rpd3 Protein
CAS No.	144714-44-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Recombinant Rpd3 Protein; HDAC1 (Homolog); Histone Deacetylase Rpd3; Yeast RPD3 Protein; KDAC1 (Yeast); Lysine Deacetylase Rpd3; Transcriptional Regulator Rpd3; Class I HDAC (Yeast); RPD3L Complex Subunit; Silencing Protein Rpd3
EINECS	Contact for details

Quality Control

Our Rpd3 Protein is produced under stringent conditions to ensure high purity and consistent biological activity. Each batch undergoes rigorous quality testing, including purity assessment by SDS-PAGE and HPLC, activity validation via fluorometric or colorimetric enzyme assays, and confirmation of identity. Certificates of Analysis (COA) detailing purity, specific activity, and storage buffer composition are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive) and should be handled in a dry environment. Allow the vial to equilibrate to room temperature before opening.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 90%
Identification (Western Blot)	Positive for Rpd3/HDAC1
Specific Activity	≥ [Value] units/mg
Protein Concentration	≥ [Value] mg/mL
Buffer Composition	Tris-HCl, Glycerol, DTT, etc.
Endotoxin Level	< 1.0 EU/µg (LAL test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.