Description

5-(n-2'-(4''-Azidosalicylamidino)Ethyl-N'-Isopropyl)Amiloride is a specialized, photoactivatable derivative of the sodium channel blocker amiloride. This compound matters for researchers as it serves as a critical chemical biology tool for covalent protein labeling and cross-linking studies, enabling the investigation of ion channel structure and function. It is primarily needed by pharmaceutical R&D teams, academic biochemistry laboratories, and proteomics researchers focused on membrane protein dynamics and drug discovery.

Application

• Photoaffinity Labeling: Used as a covalent probe to identify and characterize amiloride-sensitive sodium channels (ENaC) and other protein targets in biological membranes.
• Proteomics Research: Enables the capture and isolation of interacting proteins for mass spectrometry analysis to map protein-protein interaction networks.
• Structural Biology Studies: Aids in determining the binding sites and conformational changes of ion channels and transporters through cross-linking experiments.
• Drug Discovery Platform: Serves as a key intermediate or tool compound in high-throughput screening campaigns and mechanism-of-action studies for novel diuretic or anti-hypertensive agents.
• Bioconjugation: The azido group allows for subsequent "click chemistry" reactions, facilitating the attachment of fluorophores, biotin, or other tags for detection and purification.

Basic Information

Product Name	5-(n-2'-(4''-Azidosalicylamidino)Ethyl-N'-Isopropyl)Amiloride
CAS No.	144176-47-6
Molecular Formula	C24H28N8O4
Molecular Weight	492.53 g/mol
Synonyms	5-(N-2-(4-Azidosalicylamido)Ethyl-N-Isopropyl)Amiloride; Benzamil Azidosalicylamide Derivative; Photoactivatable Amiloride; Amiloride Photoaffinity Label; 5-(N-[2-(4-Azido-2-hydroxybenzamido)ethyl]-N-isopropyl)amiloride; APA; Azido Photoaffinity Amiloride Analog
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards for research chemicals. Every batch is characterized by advanced analytical techniques to confirm identity and purity, providing researchers with reliable and reproducible results. Certificates of Analysis (COA) detailing HPLC purity, NMR, and mass spectrometry data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Due to its strictly light-sensitive and hygroscopic nature, this material must be stored in amber vials or foil-wrapped containers under an inert atmosphere (e.g., argon or nitrogen) at -20°C or below for long-term stability. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to light brown solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.