Description

24(Rs)-Hydroxycholesterol-D7 is a high-purity, deuterium-labeled isotopologue of the endogenous oxysterol 24(S)-hydroxycholesterol, a key biomarker for cholesterol metabolism in the brain. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring unparalleled accuracy and reliability in analytical workflows. It is critically needed by pharmaceutical R&D laboratories, clinical research organizations, and academic institutions focused on lipidomics, neurodegenerative disease research, and drug metabolism studies.

Application

• Quantitative Mass Spectrometry Internal Standard: Primary application as a deuterated internal standard for the precise LC-MS/MS quantification of endogenous 24(S)-hydroxycholesterol in biological matrices (plasma, cerebrospinal fluid, brain tissue).
• Biomarker Research: Enables accurate study of cholesterol turnover and brain cholesterol homeostasis, crucial for research into Alzheimer's disease, multiple sclerosis, and other neurological disorders.
• Drug Discovery & Development: Used in pharmacokinetic and pharmacodynamic studies to assess the impact of drug candidates on cholesterol 24-hydroxylase (CYP46A1) activity and central nervous system cholesterol metabolism.
• Clinical Diagnostics Assay Development: Serves as a critical reference material in the development and validation of robust clinical diagnostic assays for oxysterol profiling.
• Metabolic Pathway Tracing: Facilitates advanced metabolic flux analysis studies to elucidate complex pathways of cholesterol metabolism and elimination.
• Quality Control & Method Validation: Employed in analytical laboratories for method development, calibration, and ensuring compliance with guidelines like FDA Bioanalytical Method Validation.

Basic Information

Product Name	24(Rs)-Hydroxycholesterol-D7
CAS No.	144154-78-9
Molecular Formula	C₂₇H₃₉D₇O₃
Molecular Weight	~ 419.7 g/mol
Synonyms	(24R/S)-Hydroxycholesterol-d7; 24-Hydroxycholesterol-d7; 24(R/S)-HC-d7; Cholest-5-ene-3β,24-diol-d7; 24S-Hydroxycholesterol Deuterated Standard; 24(S)-Hydroxy-cholesterol-d7; CYP46A1 Metabolite-d7; Cerebrosterol-d7
EINECS	Contact for details

Quality Control

Every batch of 24(Rs)-Hydroxycholesterol-D7 is manufactured and analyzed under strict quality systems. We guarantee high chemical and isotopic purity, confirmed by advanced analytical techniques including NMR and high-resolution mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, isotopic enrichment, and solvent content is provided with each shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98% (Chemical Purity)
Isotopic Enrichment	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.