Description

5-Bromo-6-Chloro-3-Indolyl-D-Glucuronide Cyclohexylammonium Salt is a high-purity chromogenic substrate specifically designed for enzymatic detection and analysis. This compound is critical for researchers and quality control laboratories requiring precise and sensitive visualization of β-glucuronidase activity. It is primarily utilized in molecular biology, diagnostic assay development, and microbiological research for applications such as blue-white screening and reporter gene assays.

Application

• Molecular Biology Research: As a key chromogenic substrate for detecting β-glucuronidase (GUS) reporter gene expression in plant and bacterial systems.
• Diagnostic Assay Development: Used in the formulation of culture media and test kits for the specific identification of E. coli and other β-glucuronidase-producing microorganisms.
• Microbiological Analysis: Employed in agar plates for blue-white colony screening, enabling rapid visual differentiation in cloning experiments.
• Enzyme Activity Studies: Serves as a specific substrate for kinetic studies and the quantification of β-glucuronidase enzyme activity in various samples.
• Histochemistry: Used in tissue staining protocols to localize GUS enzyme activity with high specificity.
• Pharmaceutical QC: Applied in quality control testing within biotechnology and pharmaceutical manufacturing where GUS is used as a marker.

Basic Information

Item	Details
Product Name	5-Bromo-6-Chloro-3-Indolyl-D-Glucuronide Cyclohexylammonium Salt
CAS No.	144110-43-0
Molecular Formula	C20H24BrClN2O7 • C6H13N
Molecular Weight	Approx. 583.78 g/mol (for the salt)
Synonyms	X-Glucuronide, Cyclohexylammonium Salt; 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid cyclohexylammonium salt; BCIG; X-Gluc; 5-Bromo-6-chloro-3-indolyl-β-D-glucopyranosiduronic acid cyclohexylamine salt; Magenta-β-D-glucuronide (related); GUS Substrate
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indolyl-D-Glucuronide Cyclohexylammonium Salt is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the stringent requirements for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.