Description

5-Bromo-6-Chloro-3-Indolyl β-D-Glucuronide is a chromogenic substrate specifically designed for the detection of β-glucuronidase enzyme activity. This compound is critical for molecular biology and diagnostic applications, where it serves as a key reagent in histochemical staining, microbiological assays, and reporter gene systems. It is primarily utilized by research institutions, pharmaceutical developers, and diagnostic kit manufacturers for applications in gene expression studies, bacterial identification, and enzyme-linked detection methods.

Application

• Histochemical Staining: Used as a substrate for β-glucuronidase (GUS) in plant molecular biology and transgenic research to visualize gene expression patterns.
• Microbiological Detection: Employed in selective media for the identification and enumeration of E. coli and other β-glucuronidase-positive bacteria in food, water, and clinical samples.
• Reporter Gene Assays: Serves as a key reagent in enzymatic reporter gene systems (GUS assays) for studying promoter activity and gene regulation.
• Diagnostic Kits: Incorporated into commercial diagnostic kits and test strips for the rapid detection of specific bacterial contaminants.
• Enzyme Characterization: Used in biochemical research to study the kinetics and inhibition of β-glucuronidase enzymes.
• Cell-Based Screening: Applied in high-throughput screening platforms to identify modulators of β-glucuronidase activity for drug discovery.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indolyl β-D-Glucuronide
CAS No.	144110-42-9
Molecular Formula	C14H15BrClNO7
Molecular Weight	424.63 g/mol
Synonyms	5-Bromo-6-chloro-3-indolyl-β-D-glucopyranosiduronic acid; X-Glucuronide; BCIG; 5-Bromo-6-chloro-1H-indol-3-yl β-D-glucopyranosiduronic acid; Magenta-Gluc; 5-Br-6-Cl-3-indolyl-β-D-glucuronide; GUS Substrate (5-Bromo-6-Chloro-3-Indolyl); Salmagluc
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indolyl β-D-Glucuronide is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is analyzed to confirm identity, purity, and performance in enzymatic assays. A comprehensive Certificate of Analysis (COA) detailing specifications such as HPLC purity, water content, and enzymatic activity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Enzymatic Activity	Passes performance test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.