Description

Neurofilament Protein Nf 60 is a specific neurofilament subunit protein, a key structural component of the neuronal cytoskeleton. This high-purity protein is essential for advanced research into neurodegenerative diseases, axonal transport, and neuronal development. It is a critical reagent for biotechnology companies, diagnostic developers, and academic research institutions focused on neuroscience, biomarker discovery, and neurotoxicity studies.

Application

• Biomarker Research & Assay Development: Used as a standard and antigen in immunoassays (ELISA, Western Blot) to detect and quantify neurofilament levels, a key biomarker for neuronal damage in conditions like ALS, Alzheimer's, and multiple sclerosis.
• Neurodegenerative Disease Studies: Serves as a critical tool for investigating the mechanisms of axonal degeneration, protein aggregation, and cytoskeletal disruption in disease models.
• Diagnostic Kit Manufacturing: Acts as a purified antigen for the production and calibration of in-vitro diagnostic (IVD) kits and companion diagnostics for neurological disorders.
• Antibody Production & Validation: Used to immunize animals for generating specific monoclonal or polyclonal antibodies against neurofilament proteins.
• Cell Biology & Neuroscience Research: Employed in studies of neuronal cell culture, axonal growth, and the structural integrity of the cytoskeleton.
• Neurotoxicity Screening: Applied in pharmaceutical and chemical safety testing to assess compound-induced damage to neuronal structures.

Basic Information

Product Name	Neurofilament Protein Nf 60
CAS No.	143221-01-6
Molecular Formula	Contact for details
Molecular Weight	~60 kDa (Protein)
Synonyms	Neurofilament 60; NF-60; Neurofilament Medium Subunit; NF-M; Neurofilament Protein, Medium Polypeptide; 160 kDa Neurofilament Protein; Neurofilament Triplet M Protein; Neuronal Intermediate Filament Protein M
EINECS	Contact for details

Quality Control

Our Neurofilament Protein Nf 60 is produced and tested under stringent quality management systems. Each batch is characterized for identity, purity, and biological activity using advanced analytical techniques including SDS-PAGE, Western Blot, and immunoassay. Certificates of Analysis (COA) detailing purity (typically >95% by SDS-PAGE), concentration, and endotoxin levels are provided to ensure it meets the exacting standards of research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted protein aliquots should be stored at -70°C to -80°C to prevent degradation. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Western Blot)	Positive reaction with anti-NF-M antibodies
Purity (SDS-PAGE)	> 95%
Protein Concentration	As per COA (e.g., 1 mg/vial)
Endotoxin Level	< 1.0 EU/μg (by LAL assay)
Biological Activity	Functional in specified immunoassays
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.