Description

5'-O-(4,4'-Dimethoxytrityl)-N2-Isobutyryl-8-Oxo-2'-Deoxyguanosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is a highly specialized, protected phosphoramidite monomer essential for the automated solid-phase synthesis of oligonucleotides. This compound matters because it enables the precise and efficient incorporation of a modified guanine base, 8-oxo-2'-deoxyguanosine, which is a critical biomarker for oxidative DNA damage in research. It is primarily needed by biotechnology and pharmaceutical companies, academic research institutions, and diagnostic developers focused on studying DNA oxidation, mutagenesis, and developing probes for genetic analysis. The product is supplied under strict quality control to ensure optimal coupling efficiency and yield in oligonucleotide synthesis.

Application

• Synthesis of oligonucleotide probes containing 8-oxo-dG for the study of oxidative stress and DNA damage.
• Research tool in mutagenesis studies to understand the mutagenic potential of 8-oxoguanine lesions.
• Production of modified antisense oligonucleotides (ASOs) or siRNAs for therapeutic research applications.
• Development of diagnostic assays and sensors for detecting oxidative DNA damage biomarkers.
• Solid-phase DNA synthesis for creating site-specifically modified DNA strands used in structural biology.
• Preparation of internal standards for mass spectrometry-based quantification of oxidized nucleotides.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-N2-Isobutyryl-8-Oxo-2'-Deoxyguanosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite
CAS No.	143060-53-1
Molecular Formula	C47H52N7O9P
Molecular Weight	889.93 g/mol
Synonyms	8-Oxo-dG Phosphoramidite; 8-Oxo-2'-deoxyguanosine Phosphoramidite; 8-Oxo-dG-CE Phosphoramidite; DMTr-8-oxo-dG(iBu)-CE Phosphoramidite; 5'-O-DMT-N2-Isobutyryl-8-oxo-2'-deoxyguanosine,3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 8-Oxo-7,8-dihydro-2'-deoxyguanosine Phosphoramidite; Guanosine, 2'-deoxy-8-oxo-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite], 5'-[bis(4-methoxyphenyl)phenylmethyl] 2-(2-methyl-1-oxopropoxy) derivative
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-N2-Isobutyryl-8-Oxo-2'-Deoxyguanosine Phosphoramidite is manufactured under controlled conditions to meet the exacting standards of oligonucleotide synthesis. Each batch undergoes rigorous quality testing including HPLC for purity and identity confirmation (¹H NMR, ³¹P NMR) to ensure optimal performance and coupling efficiency. Certificates of Analysis (COA) detailing purity, moisture content, and spectroscopic data are available upon request to support your research and cGMP-compliant production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (³¹P NMR)	Conforms
Purity (HPLC)	≥ 97.0%
Moisture (Karl Fischer)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.