Description

5'-(Tetrahydrogen Triphosphate), 2'-o-Methyl-Uridine is a chemically modified nucleoside triphosphate analog, specifically a 2'-O-methyl derivative of uridine triphosphate. This modification confers enhanced nuclease resistance and stability, making it a critical building block for advanced oligonucleotide synthesis. It is essential for research and development in the fields of molecular biology, diagnostics, and therapeutic oligonucleotide manufacturing, particularly for antisense, siRNA, and aptamer technologies.

Application

• Therapeutic Oligonucleotide Synthesis: A key raw material for the solid-phase synthesis of antisense oligonucleotides (ASOs), small interfering RNA (siRNA), and aptamers with improved metabolic stability.
• Diagnostic Probe Development: Used in the enzymatic or chemical synthesis of labeled probes for fluorescence in situ hybridization (FISH) and microarray analysis.
• RNA Biochemistry Research: Essential for studying RNA structure, function, and RNA-protein interactions where nuclease-resistant analogs are required.
• Enzymatic RNA Labeling: Serves as a substrate for RNA polymerases (e.g., T7 RNA polymerase) to incorporate modified nucleotides into RNA transcripts.
• Next-Generation Sequencing (NGS): Potential application in the development of novel sequencing chemistries and modified nucleotide libraries.
• Biocatalysis & Enzyme Studies: Used as a specialized cofactor or inhibitor in enzymatic studies involving nucleotide-dependent enzymes.

Basic Information

Product Name	5'-(Tetrahydrogen Triphosphate), 2'-o-Methyl-Uridine
CAS No.	143028-99-3
Molecular Formula	C10H17N2O15P3
Molecular Weight	522.15 g/mol
Synonyms	2'-O-Methyluridine-5'-triphosphate; 2'-O-Me-UTP; 2'-O-Methyl-UTP; Uridine 5'-(tetrahydrogen triphosphate), 2'-O-methyl-; 2'-Methoxyuridine triphosphate; 2'-OMe-UTP; 2'-O-Methyluridine triphosphate sodium salt; 2'-O-Methyl-5'-UTP
EINECS	Contact for details

Quality Control

Our 5'-(Tetrahydrogen Triphosphate), 2'-o-Methyl-Uridine is manufactured under strict quality systems suitable for research and pharmaceutical development. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets stringent specifications. Certificates of Analysis (COA) with detailed chromatographic data are provided for traceability and quality assurance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Triphosphate Content (NMR/HPLC)	≥ 85%
Di- & Monophosphate Impurities (HPLC)	Reported on COA
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.