Description

Hsp104 Protein CAS NO 143012-44-6 is a highly conserved molecular chaperone belonging to the AAA+ (ATPases Associated with diverse cellular Activities) protein superfamily. This protein plays a critical role in cellular proteostasis by mediating the disassembly and reactivation of aggregated proteins, a function essential for stress tolerance and cellular viability. It is a vital research tool for scientists in the fields of molecular biology, biochemistry, and neurodegenerative disease research, providing insights into protein misfolding disorders such as Alzheimer's, Parkinson's, and Huntington's disease.

Application

• Biochemical Research: Used as a key reagent in studies of protein disaggregation, refolding mechanisms, and chaperone network functions.
• Neurodegenerative Disease Models: Employed in in vitro and cellular assays to investigate the dissolution of amyloid fibrils and other pathogenic protein aggregates.
• Yeast Genetics and Cell Biology: Essential for studying stress response pathways in Saccharomyces cerevisiae, where Hsp104 is natively expressed.
• Drug Discovery Screening: Serves as a target in high-throughput screens for compounds that modulate chaperone activity, with potential therapeutic applications.
• Protein Engineering: Utilized in developing engineered chaperone systems for biotechnology applications, including improving recombinant protein solubility and yield.
• Prion Biology Studies: Investigated for its unique ability to propagate and eliminate yeast prions, providing a model for mammalian prion diseases.

Basic Information

Product Name	Hsp104 Protein
CAS No.	143012-44-6
Molecular Formula	Contact for details
Molecular Weight	~104 kDa (monomer)
Synonyms	Heat shock protein 104; HSP104; Heat shock protein 104 kDa; Protein disaggregase Hsp104; EC 3.6.4.10; AAA ATPase Hsp104; Chaperone protein Hsp104; Thermotolerance protein 1
EINECS	Contact for details

Quality Control

Our Hsp104 Protein is produced and purified under stringent conditions to ensure high biological activity and batch-to-batch consistency. Each lot is subjected to a comprehensive suite of analytical tests, including SDS-PAGE for purity assessment, Western Blot for identity confirmation, and functional ATPase/disaggregation assays. A detailed Certificate of Analysis (COA) documenting purity, concentration, buffer composition, and specific activity is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle using standard laboratory precautions for biological materials.

Specification

Item	Specification
Appearance	Clear or slightly opalescent liquid
Identification (Western Blot)	Positive for Hsp104
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	≥ 0.5 mg/mL
Buffer	Specified on COA (e.g., Tris, HEPES with stabilizers)
Endotoxin Level	< 1.0 EU/µg (for cell culture grades)
Biological Activity (ATPase/Disaggregation)	Reported on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.