Description

5'-Carboxyl-2',7'-Dichlorodihydrofluorescein is a key chemical intermediate and precursor used in the synthesis of advanced fluorescent probes and dyes. This compound matters for its role in creating highly sensitive detection reagents, particularly for reactive oxygen species (ROS) assays in biological research. It is primarily needed by manufacturers and research institutions in the life sciences, pharmaceutical development, and diagnostic kit production sectors. Its functional carboxyl group allows for further conjugation, making it a versatile building block for custom fluorescent labels and sensors.

Application

• Primary Precursor for H2DCFDA: The essential starting material for synthesizing 2',7'-Dichlorodihydrofluorescein diacetate (H2DCFDA), a widely used cell-permeable probe for detecting intracellular reactive oxygen species (ROS).
• Fluorescent Probe Development: Used in R&D to create novel fluorescent dyes and biosensors for oxidative stress measurement, cellular imaging, and apoptosis studies.
• Diagnostic Reagent Manufacturing: Incorporated into the production of reagents for research-use-only (RUO) and in vitro diagnostic (IVD) kits that measure oxidative activity.
• Bioconjugation Chemistry: The carboxyl group enables covalent attachment to proteins, peptides, antibodies, or nanoparticles to create targeted fluorescent conjugates for assay development.
• Pharmaceutical Research: Employed in drug discovery programs to screen for antioxidant compounds or to study the mechanisms of diseases involving oxidative damage.
• Quality Control Reference Standard: Serves as a critical reference standard and impurity marker in the quality control of final fluorescent probe batches.

Basic Information

Product Name	5'-Carboxyl-2',7'-Dichlorodihydrofluorescein
CAS No.	142975-81-3
Molecular Formula	C21H12Cl2O7
Molecular Weight	447.23 g/mol
Synonyms	5(6)-Carboxy-2',7'-Dichlorodihydrofluorescein; 5(6)-Carboxy-2',7'-Dichlorofluorescein (reduced form); DCFH-COOH; 2',7'-Dichloro-3',6'-dihydroxy-5(6)-carboxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]; 5(6)-Carboxy-2',7'-dichloro-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthene]; Precursor to H2DCFDA; Reduced Carboxy-Dichlorofluorescein
EINECS	Contact for details

Quality Control

Our 5'-Carboxyl-2',7'-Dichlorodihydrofluorescein is produced under strict quality management systems to ensure batch-to-batch consistency and high purity, critical for sensitive assay performance. Each lot undergoes rigorous analytical testing including HPLC for purity, NMR and MS for structural confirmation, and residual solvent analysis. Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We support compliance with relevant research and manufacturing standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. The material is light-sensitive and easily oxidized; prolonged exposure to air, light, or elevated temperatures should be avoided to maintain stability and performance. Keep the container in a dry, cool, and well-ventilated place.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Related Substances (HPLC)	Individual impurity ≤ 1.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.