Description

Bub3 Protein CAS NO 142008-15-9 is a high-purity, biologically active protein essential for advanced life science research and development. This product is critical for studies involving cell cycle regulation, signal transduction, and protein-protein interaction networks. It is primarily utilized by researchers and process developers in the pharmaceutical, biotechnology, and academic sectors for target validation, assay development, and biochemical studies.

Application

• Biochemical Research: Used as a key reagent in enzymatic assays, protein interaction studies, and structural biology to elucidate biological pathways.
• Drug Discovery & Target Validation: Serves as a critical target or tool protein in high-throughput screening (HTS) campaigns and mechanism-of-action studies for novel therapeutics.
• Diagnostic Development: Employed in the development and calibration of immunoassays, biosensors, and other diagnostic platforms requiring highly specific protein components.
• Cell Biology Studies: Utilized in cellular transfection and microinjection experiments to study gene function, cell cycle progression, and apoptosis.
• Protein Standard & Control: Acts as a purity standard and positive control in Western blotting, ELISA, and mass spectrometry-based proteomic analyses.
• Enzyme Substrate or Cofactor: Functions in specialized enzymatic reactions for studying post-translational modifications like ubiquitination.

Basic Information

Product Name	Bub3 Protein
CAS No.	142008-15-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	BUB3 Mitotic Checkpoint Protein; BUB3, Budding Uninhibited by Benzimidazoles 3 Homolog; BUB3L; hBUB3; Mitotic Checkpoint Protein BUB3; BUB3 Human; Recombinant BUB3 Protein; BUB3 (Yeast) Homolog
EINECS	Contact for details

Quality Control

Our Bub3 Protein is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and biological activity. Each lot undergoes comprehensive analysis including purity assessment, activity assays, and endotoxin testing. A Certificate of Analysis (COA) detailing specific results for identity, purity, and activity is provided with every shipment, supporting compliance with research and development standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Single band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Content	Determined by UV absorbance
Biological Activity	Assessed by functional binding assay
Endotoxin Level	< 1.0 EU/µg
Solubility	Reconstitutes in specified buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.