Description

Bub2 Protein CAS NO 142008-14-8 is a high-purity recombinant protein, a key regulatory component in the spindle assembly checkpoint (SAC) pathway essential for accurate chromosome segregation during cell division. Its primary value lies in enabling critical biochemical and cellular research into mitotic regulation, genome stability, and cancer mechanisms. This product is indispensable for researchers and developers in academic institutions, pharmaceutical R&D, and biotechnology companies focused on cell cycle studies, drug discovery for oncology targets, and diagnostic assay development.

Application

• Biochemical Research: Used as a critical reagent in kinase activity assays and protein-protein interaction studies to elucidate the spindle assembly checkpoint mechanism.
• Drug Discovery & Screening: Serves as a target protein in high-throughput screening (HTS) platforms to identify novel small molecule inhibitors for cancer therapeutics.
• Antibody Production & Validation: Acts as a high-quality antigen for generating and characterizing specific monoclonal and polyclonal antibodies.
• Cellular & Molecular Biology: Employed in microinjection and transfection experiments to study Bub2 function and localization in live cells.
• Diagnostic Development: Utilized as a reference standard in the development of ELISA and other immunoassays for research purposes.
• Enzymology Studies: Essential for kinetic analysis and understanding the regulatory role of Bub2 in the mitotic exit network (MEN).

Basic Information

Product Name	Bub2 Protein
CAS No.	142008-14-8
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Bub2; Mitotic checkpoint protein BUB2; BUB2, mitotic checkpoint serine/threonine-protein kinase; BUB2 budding uninhibited by benzimidazoles 2 homolog; Recombinant Bub2 Protein; Yeast Bub2 Protein; SAC protein Bub2; Cell cycle checkpoint protein Bub2
EINECS	Contact for details

Quality Control

Our Bub2 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, endotoxin level determination, and functional activity validation in relevant biochemical assays. We adhere to ISO 9001 quality management principles. Comprehensive Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Content (UV)	≥ 90%
Biological Activity	Confirmed by functional assay
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.