Description

Boc-[15N]Val-Oh is a stable isotope-labeled derivative of the amino acid valine, where the nitrogen atom is enriched with the 15N isotope and the α-amino group is protected by a tert-butoxycarbonyl (Boc) group. This compound is a critical building block in the synthesis of isotopically labeled peptides and proteins for advanced research applications, providing a non-radioactive tracer for mass spectrometry and NMR spectroscopy studies. It is essential for scientists in pharmaceutical R&D, proteomics, and metabolic research who require high-purity, chemically defined labeled intermediates to investigate protein structure, function, and dynamics.

Application

• Isotope-Labeled Peptide Synthesis: Serves as a key precursor for the solid-phase or solution-phase synthesis of peptides containing 15N-labeled valine residues.
• Biomolecular NMR Spectroscopy: Used to incorporate 15N labels into proteins, enabling detailed structural and dynamic analysis through heteronuclear NMR experiments.
• Mass Spectrometry (MS) Standards: Acts as an internal standard or reference compound in quantitative proteomics and metabolomics to ensure accurate mass measurement and quantification.
• Metabolic Pathway Tracing: Employed in metabolic flux analysis studies to track the incorporation of nitrogen in biological systems.
• Pharmaceutical Research: Utilized in the development and characterization of peptide-based drug candidates, where isotopic labeling aids in pharmacokinetic and stability studies.
• Chemical Biology Probes: Incorporated into activity-based probes or affinity tags to study enzyme function and protein-protein interactions.

Basic Information

Product Name	Boc-[15N]Val-Oh
CAS No.	141509-91-3
Molecular Formula	C10H1915NO4
Molecular Weight	218.26 g/mol (for 15N isotopologue)
Synonyms	N-(tert-Butoxycarbonyl)-L-valine-15N; Boc-L-valine-15N; (2S)-2-[(tert-Butoxycarbonyl)(15N)amino]-3-methylbutanoic acid; (S)-2-((tert-Butoxycarbonyl)(15N)amino)-3-methylbutanoic acid; N-Boc-L-valine-15N; tert-Butyloxycarbonyl-L-valine-15N; Boc-Val-15N-OH; Isotopically labeled Boc-Val-OH
EINECS	Contact for details

Quality Control

Our Boc-[15N]Val-Oh is manufactured under strict quality control protocols to ensure exceptional isotopic and chemical purity for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as isotopic enrichment, chemical purity, chiral purity, and impurity profiles. We adhere to cGMP principles where applicable to support regulatory filings. Certificates of Analysis are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a cool, dry place. This product is hygroscopic (moisture-sensitive). For long-term storage under inert atmosphere, consider sealing under argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment (15N)	≥ 98 atom %
Chiral Purity (HPLC)	≥ 99.0% (L-isomer)
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.