Description

Mad2 Protein CAS NO 141349-76-0 is a highly purified, recombinant protein critical for the study of cell cycle regulation and the spindle assembly checkpoint. This protein is essential for researchers investigating mechanisms of chromosome segregation, genomic stability, and the molecular basis of diseases like cancer. It is primarily utilized by academic, pharmaceutical, and biotechnology laboratories engaged in advanced biochemical, biophysical, and cell biology research.

Application

• Biochemical Research: Used as a key reagent in in vitro binding assays to study protein-protein interactions within the mitotic checkpoint complex (MCC).
• Drug Discovery Screening: Serves as a target protein in high-throughput screening platforms to identify novel compounds that modulate the spindle assembly checkpoint for potential anti-cancer therapeutics.
• Structural Biology: Employed in X-ray crystallography and cryo-electron microscopy studies to elucidate the three-dimensional architecture of checkpoint complexes.
• Enzyme Activity Studies: Utilized in kinetic assays to understand its role in the inhibition of the Anaphase-Promoting Complex/Cyclosome (APC/C).
• Antibody Production & Validation: Acts as a high-purity antigen for generating and characterizing specific monoclonal or polyclonal antibodies.
• Educational & Diagnostic Development: Supports advanced teaching in molecular biology and serves as a component in developing diagnostic tools for chromosomal instability syndromes.

Basic Information

Product Name	Mad2 Protein
CAS No.	141349-76-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	MAD2L1 Protein; Mitotic Arrest Deficient 2 Homolog Protein; MAD2 (Mitotic Arrest Deficient-Like 1); HsMAD2; Recombinant Human MAD2 Protein; MAD2 Mitotic Checkpoint Protein; p55CDC Protein
EINECS	Contact for details

Quality Control

Our Mad2 Protein is produced under stringent conditions to ensure high batch-to-batch consistency and biological activity. Each lot undergoes rigorous analytical testing, including purity assessment by SDS-PAGE and HPLC, confirmation of identity by mass spectrometry, and functional activity validation in appropriate binding or inhibition assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, buffer composition, and storage conditions is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. Thaw on ice and keep on ice or at 4°C during use. The product is stable under these conditions for the period stated on the label.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Identification (Mass Spec)	Conforms
Protein Concentration	≥ 0.5 mg/mL
Buffer Composition	Specified on COA
Endotoxin Level	< 1.0 EU/µg
Biological Activity	Functional assay confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.