Description

Boc-[15N]Ala-OH is a stable isotope-labeled derivative of the amino acid alanine, specifically enriched with nitrogen-15 at the α-amino position and protected at the N-terminus with a tert-butoxycarbonyl (Boc) group. This compound is a critical building block for the synthesis of isotopically labeled peptides and proteins, enabling advanced research in nuclear magnetic resonance (NMR) spectroscopy and mass spectrometry. It is essential for scientists in pharmaceutical R&D, academic biochemistry, and proteomics who require precise metabolic tracing, structural elucidation, and quantitative analysis.

Application

• Isotope-Labeled Peptide Synthesis: Serves as a key precursor for incorporating the 15N label into custom peptides for NMR studies.
• Metabolic Pathway Tracing: Used in stable isotope labeling by amino acids in cell culture (SILAC) and other metabolic labeling techniques to study protein turnover and dynamics.
• Biomolecular NMR Spectroscopy: Provides the 15N label necessary for multidimensional heteronuclear NMR experiments to determine protein structure and monitor interactions.
• Mass Spectrometry Standards: Acts as an internal standard for the accurate quantification of alanine-containing peptides in LC-MS/MS workflows.
• Pharmaceutical Research: Utilized in the development and characterization of peptide-based drug candidates and biopharmaceuticals.
• Academic Research: Fundamental tool for investigating amino acid metabolism, enzyme mechanisms, and protein folding in labeled systems.

Basic Information

Product Name	Boc-[15N]Ala-OH
CAS No.	139952-87-7
Molecular Formula	C8H1615NO4
Molecular Weight	~206.2 g/mol (for 15N isotopologue)
Synonyms	(S)-2-((tert-Butoxycarbonyl)amino)propanoic-15N acid; N-(tert-Butoxycarbonyl)-L-alanine-15N; Boc-L-alanine-15N; Boc-Ala-15N-OH; N-Boc-L-alanine-15N; tert-Butyloxycarbonyl-15N-L-alanine; 2-[(2-Methylpropan-2-yl)oxycarbonylamino]propanoic-15N acid; BOC-Ala-15N
EINECS	Contact for details

Quality Control

Our Boc-[15N]Ala-OH is manufactured under strict quality control protocols to ensure high isotopic and chemical purity for sensitive research applications. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing key parameters such as isotopic enrichment, chemical purity, and chiral purity. We adhere to industry best practices, and specifications can be tailored to meet specific research or cGMP guidelines upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. Allow the sealed container to reach ambient temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Enrichment (15N)	≥98 atom %
Optical Rotation	Specific range provided on COA
Water Content (KF)	≤0.5%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.