Description

Ydj1 Protein CAS NO 139874-78-5 is a highly purified, recombinant molecular chaperone protein belonging to the Hsp40 (DnaJ) family, essential for protein folding, translocation, and degradation processes. Its primary value lies in its critical role as a co-chaperone for Hsp70 proteins, making it an indispensable research tool for studying protein homeostasis, misfolding diseases, and cellular stress responses. This protein is vital for researchers and developers in academic research, pharmaceutical discovery, and biotechnology, particularly for in vitro assays investigating chaperone mechanisms, neurodegenerative disease models, and high-throughput screening platforms.

Application

• Biochemical Research: Used as a key reagent in in vitro protein refolding, disaggregation, and translocation assays to study Hsp70 system mechanics.
• Drug Discovery Screening: Employed in high-throughput screening (HTS) platforms to identify novel modulators of the chaperone network for therapeutic intervention.
• Neurodegenerative Disease Studies: Critical for research models focusing on protein aggregation in conditions like Alzheimer's, Parkinson's, and Huntington's diseases.
• Enzymology Studies: Serves as a co-factor to stimulate the ATPase activity of cognate Hsp70 partners in kinetic studies.
• Structural Biology: Provides a pure, well-characterized protein for X-ray crystallography, NMR, and cryo-EM studies of chaperone-substrate complexes.
• Diagnostic Development: Potential use as a component in diagnostic assays for detecting chaperone-related biomarkers or functional deficiencies.

Basic Information

Product Name	Ydj1 Protein
CAS No.	139874-78-5
Molecular Formula	Contact for details
Molecular Weight	~44 kDa (monomer, species-dependent)
Synonyms	YDJ1; DnaJ homolog subfamily A member 1; Hsp40; Molecular chaperone Ydj1; Saccharomyces cerevisiae Ydj1p; Recombinant Ydj1; DnaJ protein Ydj1; Chaperone protein YDJ1; ATX2 homolog; MSJ-1 homolog; HDJ2 homolog (mammalian); DNAJA1 homolog
EINECS	Contact for details

Quality Control

Our recombinant Ydj1 Protein is produced under stringent conditions and undergoes rigorous quality testing to ensure high purity, identity, and functional activity. Each batch is characterized by multiple analytical methods to guarantee consistency for research applications. Certificates of Analysis (COA) detailing purity, concentration, endotoxin levels, and functional assay data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized protein at -20°C or below. Reconstituted protein aliquots should be stored at -70°C to -80°C and avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use in a low-humidity environment.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~44 kDa
Purity (HPLC)	≥ 95%
Protein Content (BCA)	≥ 90%
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity (ATPase Assay)	Stimulates Hsp70 ATPase activity
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.