Description

Oncogene Protein Cot CAS NO 139691-74-0 is a high-purity, recombinant protein derived from the Cot oncogene, a serine/threonine kinase involved in critical cellular signaling pathways. This product is essential for researchers and developers in the life sciences sector, providing a reliable and well-characterized reagent for studying MAPK/ERK pathway activation, cell proliferation, and oncogenesis. It is primarily utilized by biotechnology and pharmaceutical companies engaged in target validation, drug discovery, and diagnostic assay development.

Application

• Biochemical Research: Used as a key reagent in kinase activity assays and signal transduction studies.
• Drug Discovery & Screening: Serves as a target protein for high-throughput screening (HTS) to identify novel kinase inhibitors.
• Diagnostic Development: Employed as a standard or coating antigen in the development of ELISA kits for cancer biomarker detection.
• Cell Biology Studies: Applied in cellular assays to investigate the role of the Cot oncogene in inflammation, apoptosis, and tumor progression.
• Antibody Production & Validation: Used as an immunogen for generating specific monoclonal or polyclonal antibodies.
• Proteomics & Protein Interaction Analysis: A critical component in pull-down assays and mass spectrometry to map protein-protein interaction networks.

Basic Information

Product Name	Oncogene Protein Cot CAS NO 139691-74-0
CAS No.	139691-74-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	COT Oncogene; Cancer Osaka Thyroid Oncogene; MAP3K8; Tumor Progression Locus 2 (TPL2); ESTF; Oncogene COT; Oncogene Tpl-2; Cot/TPL2 Protein; Serine/Threonine-Protein Kinase COT; EC 2.7.11.25
EINECS	Contact for details

Quality Control

Our Oncogene Protein Cot is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes comprehensive analytical testing, including SDS-PAGE for purity assessment, Western Blot for specificity, and functional kinase activity assays. We provide full traceability and Certificates of Analysis (COA) detailing purity, concentration, endotoxin levels, and biological activity. Our quality system is designed to support research and pre-clinical development under ISO 13485 guidelines.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Purity (SDS-PAGE)	≥ 95%
Identification (Western Blot)	Positive
Biological Activity	Confirmed by in vitro kinase assay
Endotoxin Level	< 1.0 EU/µg
Protein Concentration	By UV absorbance
Solubility	Reconstitutes in suitable buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.