Description

Athb-1 Homeodomain Protein is a purified recombinant protein derived from the Arabidopsis thaliana homeobox-leucine zipper protein family. This high-purity protein is essential for fundamental research in plant molecular biology and developmental genetics. It serves as a critical reagent for scientists studying transcription factor mechanisms, DNA-protein interactions, and gene regulation pathways. Researchers in academic, biotechnology, and agricultural sectors require this specific protein for advanced in vitro assays, including electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation (ChIP) studies.

Application

• DNA-Binding Studies: Used in Electrophoretic Mobility Shift Assays (EMSAs) to characterize specific DNA sequence recognition and binding affinity of the homeodomain.
• Transcription Factor Research: Serves as a key component in vitro transcription systems to analyze gene regulation mechanisms controlled by Athb-1.
• Protein-Protein Interaction Analysis: Employed in pull-down assays or surface plasmon resonance (SPR) to identify interacting partner proteins.
• Antibody Production & Validation: Acts as a high-quality antigen for generating and testing specificity of polyclonal or monoclonal antibodies.
• Enzymatic & Functional Assays: Utilized in activity assays to study the functional impact of the homeodomain on downstream biological processes.
• Structural Biology: Provides material for X-ray crystallography or NMR spectroscopy to determine the three-dimensional protein structure.
• Educational & Diagnostic Kit Development: Incorporated into research kits for teaching advanced molecular biology techniques or developing diagnostic tools.

Basic Information

Product Name	Athb-1 Homeodomain Protein
CAS No.	139497-94-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Arabidopsis thaliana Homeobox Protein 1; Athb-1; Homeodomain-Leucine Zipper Protein Athb-1; HD-Zip Protein Athb-1; Recombinant Athb-1 Homeodomain; ATHB-1; Homeobox-Leucine Zipper Transcription Factor
EINECS	Contact for details

Quality Control

Our Athb-1 Homeodomain Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, confirmation of identity by mass spectrometry, and functional validation in DNA-binding assays. Certificates of Analysis (COA) detailing purity, concentration, buffer composition, and specific activity are provided with every shipment to support your research compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. Thaw on ice or in a refrigerator and keep on ice during use. The specific storage buffer and recommended shelf life are provided on the product label and Certificate of Analysis.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Purity (HPLC)	≥ 90%
Concentration	≥ 0.5 mg/mL
Identification (Mass Spec)	Conforms
Endotoxin Level	< 1.0 EU/µg
Biological Activity (EMSA)	Active
Buffer	Specified on COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.