Description

7-Diethylaminocoumarin-3-Carboxylic Acid, Succinimidyl Ester is a highly reactive, amine-reactive fluorescent labeling reagent derived from the versatile coumarin dye family. This compound matters for its ability to form stable amide bonds with primary amines, facilitating the covalent tagging of biomolecules such as proteins, peptides, and antibodies with a bright, blue-fluorescent probe. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical industries for applications in fluorescence microscopy, flow cytometry, immunoassays, and biochemical sensing.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to proteins, antibodies, peptides, and oligonucleotides for detection and imaging studies.
• Proteomics and Bioanalytical Research: Used as a derivatization agent for the sensitive HPLC or capillary electrophoresis analysis of amino acids, peptides, and other amine-containing compounds.
• Diagnostic Assay Development: Labeling of capture antibodies or antigens in fluorescence-based immunoassays (e.g., ELISA, lateral flow tests) and biosensors.
• Cell Biology and Microscopy: Preparation of fluorescent probes for staining cellular components, tracking biomolecules in live or fixed cells, and fluorescence in situ hybridization (FISH).
• Material Science and Polymer Chemistry: Incorporation into polymers or surfaces to create fluorescent materials for sensing or tracking applications.
• Drug Discovery and Development: Labeling of drug candidates or biomolecular targets to study binding, distribution, and pharmacokinetics.

Basic Information

Product Name	7-Diethylaminocoumarin-3-Carboxylic Acid, Succinimidyl Ester
CAS No.	139346-57-9
Molecular Formula	C18H20N2O5
Molecular Weight	344.37 g/mol
Synonyms	7-(Diethylamino)-2-oxo-2H-chromene-3-carboxylic acid N-hydroxysuccinimide ester; 7-Diethylamino-3-carboxycoumarin NHS ester; Coumarin 343 NHS ester; C343 NHS ester; DCC-NHS; 7-Diethylaminocoumarin-3-carbonyloxysuccinimide; 7-Diethylaminocoumarin-3-carboxylic acid N-succinimidyl ester; 7-Diethylamino-3-[(2,5-dioxo-1-pyrrolidinyl)oxycarbonyl]coumarin
EINECS	Contact for details

Quality Control

Our 7-Diethylaminocoumarin-3-Carboxylic Acid, Succinimidyl Ester is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing all relevant specifications. Our quality system supports compliance with the standards required for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant (e.g., in a sealed bag with silica gel) due to its hygroscopic and moisture-sensitive nature. For long-term storage, keep the product under inert atmosphere (argon or nitrogen) to prevent degradation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange crystalline or amorphous powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMF, DMSO, acetonitrile
Extinction Coefficient	Contact for details
Fluorescence Properties	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.