Description

Mcm2 Protein, Fungal is a highly purified, recombinant protein derived from fungal expression systems, identified by CAS No. 139247-49-7. This protein is a critical component for researchers studying the molecular mechanisms of DNA replication initiation and cell cycle regulation. It is essential for biotechnology, pharmaceutical R&D, and academic laboratories focused on eukaryotic DNA replication, chromatin biology, and the development of assays for cell proliferation studies.

Application

• Biochemical Research: Used as a key reagent in in vitro studies of the pre-replication complex (pre-RC) assembly and DNA helicase loading.
• Enzymatic Activity Assays: Serves as a substrate or co-factor in assays designed to measure the activity of kinases, phosphatases, and other regulatory enzymes involved in the cell cycle.
• Protein-Protein Interaction Studies: Employed in pull-down assays, co-immunoprecipitation (Co-IP), and surface plasmon resonance (SPR) to map interactions within the replication machinery.
• Antigen for Antibody Production: High-purity protein ideal for generating specific polyclonal or monoclonal antibodies for use in Western blotting, immunohistochemistry, and ELISA.
• Drug Discovery & Screening: Target protein for high-throughput screening (HTS) platforms to identify potential therapeutic compounds that modulate DNA replication for oncology research.
• Educational & Diagnostic Kit Development: Component in advanced research kits and educational materials for molecular biology and genetics.

Basic Information

Product Name	Mcm2 Protein, Fungal
CAS No.	139247-49-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Minichromosome Maintenance Complex Component 2; MCM2; DNA replication licensing factor MCM2; CDC19; BM28; Nuclear Protein BM28; Replication Licensing Factor; Fungal Recombinant Mcm2 Protein; MCM2 Homolog
EINECS	Contact for details

Quality Control

Our Mcm2 Protein, Fungal is produced under stringent conditions to ensure high purity and batch-to-batch consistency. Each lot undergoes rigorous analytical testing, including SDS-PAGE, Western Blot, and functional activity assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, biological activity, and absence of contaminants is provided with every shipment to support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Concentration	≥ 0.5 mg/mL
Identification (Western Blot)	Positive for MCM2
Biological Activity	Confirmed by functional assay (e.g., ATPase or binding assay)
Endotoxin Level	< 1.0 EU/μg
Buffer	Specified phosphate or Tris-based buffer, pH 7.0-7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.