Description

Cdc46 Protein CAS NO 139171-68-9 is a highly purified recombinant protein, also known as the minichromosome maintenance protein 7 (MCM7), which plays a critical role in the initiation of eukaryotic DNA replication. This protein is essential for researchers and developers in the life sciences sector, serving as a key reagent for studying cell cycle regulation, DNA replication mechanisms, and genomic stability. Its high purity and biological activity make it indispensable for applications in molecular biology research, pharmaceutical development, and diagnostic assay development.

Application

• Biochemical Research: Used as a critical component in in vitro DNA replication assays to study the mechanics of the pre-replication complex.
• Drug Discovery & Development: Employed as a target protein in high-throughput screening (HTS) campaigns to identify novel therapeutics for cancer and other proliferative diseases.
• Diagnostic Development: Serves as an antigen or standard for developing immunoassays (ELISA, Western Blot) to detect MCM7 expression levels, a potential biomarker for certain cancers.
• Antibody Production & Validation: Used to immunize animals for polyclonal or monoclonal antibody generation and to validate antibody specificity.
• Enzymatic Studies: Utilized in studies investigating the ATPase and helicase activities associated with the MCM complex.
• Cell Biology: Applied in cellular imaging and localization studies to understand the spatial and temporal regulation of DNA replication factors.

Basic Information

Product Name	Cdc46 Protein
CAS No.	139171-68-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Minichromosome Maintenance Complex Component 7; MCM7; CDC47 (S. cerevisiae) Homolog; P1.1-MCM3; P85MCM; PNAS146; DNA Replication Licensing Factor MCM7; CDC46 Protein; MCM2-7 Complex Subunit; Replication Factor.
EINECS	Contact for details

Quality Control

Our Cdc46 Protein is produced under stringent conditions to ensure high purity, stability, and biological activity. Each batch undergoes comprehensive analytical testing, including SDS-PAGE, Western Blot, and functional activity assays. We provide a detailed Certificate of Analysis (COA) with every shipment, documenting purity, concentration, endotoxin levels, and buffer composition. Our quality management system adheres to ISO 9001 standards to guarantee consistency and traceability for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. Aliquot into working volumes upon receipt for optimal preservation.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Concentration	Provided on COA
Formulation	In specified storage buffer
Biological Activity	Confirmed by functional assay
Endotoxin Level	< 1.0 EU/µg
Identification (Western Blot)	Positive

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.