Description

Polypyrimidine Tract-Binding Protein CAS NO 139076-35-0 is a highly purified recombinant protein that plays a critical role in RNA metabolism, including splicing regulation, stability, and localization. This specificity makes it an essential reagent for advanced molecular biology research and diagnostic assay development. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in gene expression studies, virology, and neurological research.

Application

• Molecular Biology Research: Used as a key tool protein in studies of alternative splicing mechanisms and post-transcriptional gene regulation.
• Diagnostic Assay Development: Serves as a critical component or control in the development of ELISA, Western blot, and other immunoassays targeting RNA-binding proteins.
• Virology Studies: Employed in research on viral life cycles, particularly for viruses that interact with host cell splicing machinery.
• Neuroscience Research: Investigated for its role in neuronal differentiation and in models of neurological disorders.
• Protein-Protein Interaction Studies: Used in pull-down assays, co-immunoprecipitation, and mass spectrometry to map interactomes.
• Biopharmaceutical R&D: Acts as a target or reagent in drug discovery platforms focused on modulating RNA-protein interactions for therapeutic purposes.

Basic Information

Product Name	Polypyrimidine Tract-Binding Protein
CAS No.	139076-35-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	PTB; PTBP1; Heterogeneous Nuclear Ribonucleoprotein I (hnRNP I); pPTB; Polypyrimidine Tract Binding Protein 1; RNA-binding protein PTB; 100 kDa RNA-binding protein PTB; InRNP I
EINECS	Contact for details

Quality Control

Our Polypyrimidine Tract-Binding Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, functional activity assays, and endotoxin level checks. Certificates of Analysis (COA) detailing identity, purity, and specific activity are provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to maintain stability.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE/Western Blot)	Complies
Purity (SDS-PAGE)	> 95%
Purity (HPLC)	> 90%
Endotoxin Level	< 1.0 EU/µg
Biological Activity (Functional Assay)	Complies
Protein Concentration	As per COA
Buffer Composition	As per COA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.