Description

Cdc48 Protein CAS NO 139021-15-1 is a highly purified, recombinant protein essential for ubiquitin-dependent cellular processes. This product is critical for researchers and developers in the life sciences sector, providing a reliable and consistent reagent for advanced biochemical studies. It is primarily utilized in academic research, pharmaceutical R&D, and biotechnology applications focused on protein degradation, cell cycle regulation, and stress response pathways.

Application

• Biochemical Research: Used as a key enzyme in in vitro assays to study the mechanisms of ubiquitin-proteasome system (UPS) and endoplasmic reticulum-associated degradation (ERAD).
• Drug Discovery Screening: Serves as a target protein in high-throughput screening platforms to identify novel modulators of protein homeostasis for therapeutic development.
• Proteomics & Structural Biology: Essential for reconstituting multi-protein complexes for functional analysis, X-ray crystallography, and cryo-electron microscopy studies.
• Cell Signaling Studies: Employed to investigate its role in cell cycle control, apoptosis, and DNA damage response pathways.
• Enzyme Characterization: Used to study ATPase activity and co-factor interactions with partners like Ufd1-Npl4.
• Reagent Production: Acts as a critical component in the development of diagnostic kits and research-grade enzyme mixtures for protein degradation analysis.

Basic Information

Product Name	Cdc48 Protein
CAS No.	139021-15-1
Molecular Formula	Contact for details
Molecular Weight	~97 kDa (monomer)
Synonyms	p97; Valosin-Containing Protein (VCP); Transitional Endoplasmic Reticulum ATPase (TER ATPase); CDC48p; VAT; MB67; 15S Mg(2+)-ATPase p97 subunit
EINECS	Contact for details

Quality Control

Our Cdc48 Protein is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes comprehensive analytical testing, including assessment of purity, identity, and specific activity. A Certificate of Analysis (COA) detailing purity (by SDS-PAGE and HPLC), endotoxin levels, and functional assay results is provided with every shipment to guarantee it meets rigorous research standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -70°C or below in single-use aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Clear or slightly opalescent solution
Identification (SDS-PAGE)	Single band at ~97 kDa
Purity (HPLC)	≥ 95%
Protein Concentration	≥ 1 mg/mL
Specific Activity (ATPase)	≥ XX nmol/min/μg (Lot-specific)
Endotoxin Level	< 1.0 EU/μg
Buffer Composition	20 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 7.5

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.