Description

Bacteriophage T5 Gene A2 Protein is a highly specific endolysin enzyme derived from the T5 bacteriophage. This protein is critical for its targeted lytic activity against bacterial cell walls, making it a valuable tool in advanced microbiological and biotechnological research. It is primarily utilized by researchers and developers in the fields of molecular biology, antibacterial agent discovery, and synthetic biology for studying bacterial lysis mechanisms and developing novel biocontrol strategies.

Application

• Molecular Biology Research: Used as a model enzyme to study the mechanisms of phage-mediated bacterial lysis and cell wall degradation.
• Antibacterial Agent Development: Serves as a starting point for engineering novel enzybiotics (enzyme-based antibiotics) with targeted activity against specific bacterial pathogens.
• Bacterial Cell Disruption: Employed in laboratory protocols for the gentle and specific lysis of bacterial cells to isolate intracellular components like proteins, plasmids, or organelles.
• Synthetic Biology: A key component in designing synthetic genetic circuits and phage-based systems for controlled bacterial cell death.
• Diagnostic Tool Development: Potential use in creating biosensors or assays that detect the presence of specific bacteria based on lytic activity.
• Biofilm Dispersal Studies: Investigated for its ability to degrade the extracellular matrix of bacterial biofilms in conjunction with other agents.

Basic Information

Item	Details
Product Name	Bacteriophage T5 Gene A2 Protein
CAS No.	138898-68-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	T5 Endolysin; T5 Lysozyme; Gene A2 Product; T5 Phage Lysin; Bacteriophage T5 Lytic Enzyme; EC 3.2.1.17 (if applicable); N-acetylmuramoyl-L-alanine amidase (T5); Phage T5 A2 Protein
EINECS	Contact for details

Quality Control

Our Bacteriophage T5 Gene A2 Protein is produced and handled under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to rigorous purity analysis and functional activity assays. A comprehensive Certificate of Analysis (COA), detailing specifications such as purity (by SDS-PAGE and HPLC), protein concentration, endotoxin levels, and specific enzymatic activity, is provided to support your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. Aliquot the product upon receipt for single-use applications to maintain optimal activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (SDS-PAGE)	Single major band at expected molecular weight
Purity (HPLC)	≥ 90%
Protein Concentration	As per COA (Bradford/UV absorbance)
Specific Activity	Defined units/mg protein (Contact for details)
Endotoxin Level (LAL)	< 1.0 EU/μg
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.