Description

Rna14 Protein CAS NO 138820-57-2 is a highly purified protein component of the yeast cleavage and polyadenylation factor (CPF) complex, essential for accurate mRNA 3'-end processing. This product is critical for researchers investigating the fundamental mechanisms of eukaryotic gene expression, RNA metabolism, and transcription termination. It is primarily utilized in biochemical assays, structural biology studies, and as a key reagent for in vitro reconstitution of the mRNA processing machinery by biotechnology, pharmaceutical, and academic research institutions.

Application

• Biochemical Research: Used in in vitro transcription and polyadenylation assays to study mRNA 3'-end formation.
• Protein-Protein Interaction Studies: Essential for mapping interactions within the multi-subunit CPF and CF IA complexes.
• Enzymology: Serves as a substrate or component in studies of enzymatic activities related to RNA processing.
• Structural Biology: A key protein for X-ray crystallography, cryo-EM, and NMR studies to elucidate the architecture of the mRNA processing apparatus.
• Functional Genomics: Used to complement genetic studies in yeast to understand the phenotypic consequences of RNA14 mutations.
• Reagent Production: Acts as a critical component in the production of kits and reagents for molecular biology research.

Basic Information

Product Name	Rna14 Protein
CAS No.	138820-57-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	RNA14; Cleavage and polyadenylation factor subunit RNA14; CF IA subunit RNA14; YKL012W; Cleavage factor I subunit Rna14; mRNA 3'-end-processing protein RNA14; Rna14p
EINECS	Contact for details

Quality Control

Our Rna14 Protein is produced under stringent conditions to ensure high purity and batch-to-batch consistency. Each lot undergoes comprehensive analysis, including purity assessment by SDS-PAGE and functional activity testing in standardized polyadenylation assays. A Certificate of Analysis (COA) detailing identity, purity, concentration, and biological activity is provided with every shipment to support your research integrity.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (SDS-PAGE)	Single major band at expected molecular weight
Purity (by SDS-PAGE)	≥ 90%
Protein Concentration	As specified on COA (typically 0.5 - 1.0 mg/mL)
Biological Activity (In vitro assay)	Functional in reconstituted 3'-end processing assays
Buffer Composition	Specified on COA (typically Tris buffer, glycerol, DTT)
Endotoxin Level	< 1.0 EU/μg (if applicable)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.