Description

Mdm2 Protein CAS NO 138791-05-6 is a highly purified recombinant protein, specifically the murine double minute 2 (Mdm2) protein, which plays a critical role in the regulation of the p53 tumor suppressor pathway. This protein is an essential reagent for biochemical and biophysical studies, enabling researchers to investigate protein-protein interactions, screen for novel therapeutics, and elucidate mechanisms of oncogenesis. It is primarily utilized by pharmaceutical R&D teams, academic research laboratories, and biotechnology companies focused on cancer biology and drug discovery.

Application

• Biochemical Assay Development: Used as a key component in ELISA, fluorescence polarization (FP), and surface plasmon resonance (SPR) assays to study its interaction with p53 and other binding partners.
• High-Throughput Screening (HTS): Serves as a target protein in screening campaigns to identify small molecule inhibitors or modulators of the Mdm2-p53 interaction for anticancer drug discovery.
• Structural Biology Studies: Essential for X-ray crystallography, NMR spectroscopy, and cryo-EM to determine the three-dimensional structure of Mdm2 in complex with various ligands.
• Protein-Protein Interaction Analysis: Employed in pull-down assays, co-immunoprecipitation (Co-IP), and isothermal titration calorimetry (ITC) to quantify binding affinities and kinetics.
• Antibody Production & Characterization: Acts as a pure antigen for the generation and validation of specific monoclonal or polyclonal antibodies.
• Cell Signaling Pathway Research: Used in in vitro reconstitution experiments to model the ubiquitin ligase activity of Mdm2 and its role in p53 degradation.

Basic Information

Product Name	Mdm2 Protein
CAS No.	138791-05-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Mouse Double Minute 2 Homolog Protein; MDM2 Oncoprotein; E3 Ubiquitin-Protein Ligase Mdm2; HDM2 (Human Homolog); p53-Binding Protein Mdm2; Mdm2 (1-118) Fragment; Recombinant Mdm2 Protein; Murine Double Minute 2 Protein
EINECS	Contact for details

Quality Control

Our Mdm2 Protein is produced under stringent conditions and undergoes rigorous quality testing to ensure high purity, activity, and batch-to-batch consistency. Each lot is analyzed for identity, purity by SDS-PAGE and HPLC, endotoxin levels, and functional activity in binding assays. Certificates of Analysis (COA) detailing all specifications are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band at expected molecular weight
Purity (HPLC)	≥ 95%
Protein Content (UV)	≥ 90%
Endotoxin (LAL)	< 1.0 EU/µg
Biological Activity (Binding Assay)	Confirmed binding to p53 peptide
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.