Description

Bmi-1 Protein CAS NO 138791-04-5 is a high-purity, biologically active recombinant protein critical for research in epigenetics and stem cell biology. This protein serves as a key regulatory component of the Polycomb Repressive Complex 1 (PRC1), playing an indispensable role in modulating gene expression and cellular differentiation processes. It is an essential reagent for researchers and developers in the fields of oncology, regenerative medicine, and molecular biology, enabling advanced studies into cancer mechanisms and cellular reprogramming.

Application

• Biochemical Research: Used as a critical substrate or component in enzymatic assays to study PRC1 complex activity and histone ubiquitination.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify and validate novel inhibitors targeting the Bmi-1 oncoprotein for cancer therapy development.
• Antibody Production & Validation: Serves as a high-quality immunogen for generating specific monoclonal or polyclonal antibodies, and as a standard for antibody characterization in Western blot and ELISA.
• Stem Cell Biology Studies: Essential for in vitro experiments investigating the role of Bmi-1 in maintaining stem cell self-renewal and pluripotency.
• Diagnostic Development: Utilized as a reference standard in the development of diagnostic assays for cancers where Bmi-1 expression is a biomarker, such as certain leukemias and solid tumors.
• Cellular Reprogramming: Applied in research focused on understanding and manipulating epigenetic barriers during induced pluripotent stem cell (iPSC) generation.

Basic Information

Product Name	Bmi-1 Protein
CAS No.	138791-04-5
Molecular Formula	C129H210N44O39S
Molecular Weight	Approx. 3.1 kDa (monomeric form). Contact for details on specific construct.
Synonyms	B Lymphoma Mo-MLV Insertion Region 1 Protein; Polycomb Complex Protein BMI-1; RING Finger Protein 51 (RNF51); PCGF4 Protein; Recombinant Human Bmi-1 Protein; Bmi1 Oncoprotein; PRC1 Complex Subunit BMI1; Bmi-1.
EINECS	Contact for details.

Quality Control

Our Bmi-1 Protein is produced under stringent conditions and undergoes comprehensive analytical testing to ensure high biological activity, purity, and batch-to-batch consistency. Quality is verified through multiple orthogonal methods, including SDS-PAGE, HPLC, and functional activity assays. A detailed Certificate of Analysis (COA) is provided with each batch, documenting purity, concentration, endotoxin levels, and biological activity. Our quality system supports research requiring compliance with GMP-like guidelines for critical reagents.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized protein at -20°C or below. Upon reconstitution, aliquot and store at -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is at room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Purity (SDS-PAGE)	≥ 95%
Identification (Mass Spec)	Consistent with theoretical molecular weight
Endotoxin Level (LAL)	< 1.0 EU/µg
Biological Activity (Functional Assay)	Confirmed; specific activity provided on COA
Solubility	Soluble in aqueous buffers
Formulation	Lyophilized from a sterile, preservative-free buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.