Description

Rubredoxin, Clostridium Pasteurianum is a small, iron-sulfur redox protein isolated from the bacterium *Clostridium pasteurianum*. This compound is a crucial electron transfer agent in biochemical research, valued for its well-defined structure and role in studying fundamental redox processes. It is primarily utilized by researchers and manufacturers in the fields of enzymology, bioinorganic chemistry, and as a model system for investigating electron transport chains and metalloprotein function.

Application

• Biochemical Research: As a model protein for studying electron transfer mechanisms, iron-sulfur cluster assembly, and protein electrochemistry.
• Enzymology Studies: Used in investigations of nitrogenase and other redox-active enzyme systems where it can serve as an electron donor or acceptor.
• Bioinorganic Chemistry: Serves as a benchmark for spectroscopic studies (EPR, Mössbauer, UV-Vis) of Fe-S clusters due to its simple, single [Fe(SCys)4] site.
• Protein Engineering: A template for designing and understanding novel metalloproteins and artificial electron transfer pathways.
• Educational Tool: Used in advanced university laboratories to teach principles of metalloprotein purification, characterization, and redox chemistry.
• Reference Standard: Employed as a purity and activity standard in the production and quality control of related biochemical reagents.

Basic Information

Product Name	Rubredoxin, Clostridium Pasteurianum
CAS No.	138635-16-2
Molecular Formula	Contact for details
Molecular Weight	~6,000 Da (Approximate, varies slightly with isoform)
Synonyms	Clostridium pasteurianum Rubredoxin; C. pasteurianum Rd; Cp Rd; Iron-sulfur protein (Clostridium); Rubredoxin (C. pasteurianum); Ferredoxin (Rubredoxin type); [Fe(SCys)4] protein; Electron transfer protein; EC 1.18.1.1 (related)
EINECS	Contact for details

Quality Control

Our Rubredoxin, Clostridium Pasteurianum is produced and handled under controlled conditions to ensure high purity and consistent biological activity. Each lot undergoes rigorous analytical testing, including assessment of purity (via HPLC or electrophoresis), iron content, and specific activity. A comprehensive Certificate of Analysis (COA) detailing these specifications is provided with every shipment to support your research and quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation. For short-term use, store at 2-8°C. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Reddish-brown solid or solution
Identification (UV-Vis)	Characteristic absorbance maxima at ~280 nm, ~380 nm, and ~490 nm
Purity (HPLC or SDS-PAGE)	≥ 90% (Research Grade)
Protein Content (Bradford or UV)	≥ 90% of dry weight
Iron Content (AAS/ICP)	~1 mol Fe / mol protein
Specific Activity	Defined by electron transfer assay; reported on COA
Solubility	Soluble in aqueous buffers (e.g., Tris, phosphate), slightly basic pH

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.