Description

Lef-1 Protein CAS NO 138415-19-7 is a high-purity recombinant protein that plays a critical role as a transcription factor in the Wnt/β-catenin signaling pathway. This protein is essential for research focused on understanding cell fate determination, embryonic development, and the molecular mechanisms of certain cancers. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in advanced molecular biology, proteomics, and drug discovery programs.

Application

• Biochemical Research: Used as a key reagent in studies of the Wnt signaling cascade and its role in development and disease.
• Protein-Protein Interaction Studies: Employed in assays like EMSA (Electrophoretic Mobility Shift Assay) and co-immunoprecipitation to investigate DNA binding and partner protein interactions.
• Cell Signaling Pathway Analysis: Serves as a critical component in reconstituting and analyzing the Wnt/β-catenin pathway in vitro.
• Drug Discovery Screening: Used as a target protein in high-throughput screens for identifying potential therapeutic compounds that modulate Lef-1 activity.
• Antigen for Antibody Production: Acts as a high-quality immunogen for generating specific monoclonal or polyclonal antibodies.
• Diagnostic Development: Potential use as a standard or control in the development of diagnostic assays for conditions linked to Wnt pathway dysregulation.

Basic Information

Product Name	Lef-1 Protein
CAS No.	138415-19-7
Molecular Formula	Contact for details
Molecular Weight	Approx. 44-48 kDa (isoform-dependent)
Synonyms	Lymphoid Enhancer-binding Factor 1; TCF1-alpha; Transcription factor 1-alpha; TCF-1A; LEF1; LEF1_HUMAN; Recombinant Human LEF-1; LEF1 protein.
EINECS	Contact for details

Quality Control

Our Lef-1 Protein is produced under stringent conditions and undergoes rigorous quality testing to ensure high purity, identity, and functional activity. Each batch is analyzed for endotoxin levels and biological activity. Certificates of Analysis (COA) detailing purity (by SDS-PAGE and HPLC), concentration, and specific activity are provided to guarantee consistency and reliability for your critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, aliquot and store at ≤ -20°C. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Identification (Western Blot)	Positive
Endotoxin Level	< 1.0 EU/µg
Protein Concentration	Verified by UV absorbance
Biological Activity	Determined by functional assay (e.g., DNA-binding)
Buffer	Specified formulation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.