Description

(6-Chloro-3-Indolyl)-β-D-Glucuronide Cyclohexylammonium Salt is a chromogenic substrate widely used in molecular biology and diagnostic applications. Its core value lies in its enzymatic cleavage by β-glucuronidase (GUS), producing an insoluble, intensely colored indigo derivative ideal for histochemical staining and reporter gene assays. This compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and agricultural sectors requiring precise visualization of gene expression and enzymatic activity.

Application

• Histochemical Staining: Primary substrate for detecting β-glucuronidase (GUS) activity in plant biology, microbiology, and transgenic research.
• Reporter Gene Assays: Used in molecular biology to visualize and quantify GUS reporter gene expression in cells and tissues.
• Diagnostic Kits: A key component in enzymatic assays and kits for detecting specific bacterial strains (e.g., E. coli) that express GUS.
• Microbiological Research: Employed to study gene regulation, promoter activity, and protein localization in various organisms.
• Biotechnology & Pharmaceutical Development: Utilized in high-throughput screening and drug discovery platforms involving GUS-fusion proteins.
• Quality Control Testing: Applied in industrial fermentation and bioprocessing to monitor microbial contamination or specific enzymatic processes.

Basic Information

Product Name	(6-Chloro-3-Indolyl)-β-D-Glucuronide Cyclohexylammonium Salt
CAS No.	138182-20-4
Molecular Formula	C₂₀H₂₅ClN₂O₇ • C₆H₁₃N
Molecular Weight	521.97 g/mol
Synonyms	X-Glucuronide, Cyclohexylammonium Salt; 5-Bromo-4-chloro-3-indolyl-β-D-glucuronic acid (common analog name); 6-Chloro-3-indolyl β-D-glucopyranosiduronic acid cyclohexylammonium salt; Magenta-β-D-glucuronide (related); GUS Substrate; β-Glucuronidase Substrate; 6-Cl-IGlcU; (6-Chloro-1H-indol-3-yl)-β-D-glucopyranosiduronic acid cyclohexylamine salt
EINECS	Contact for details

Quality Control

Our (6-Chloro-3-Indolyl)-β-D-Glucuronide Cyclohexylammonium Salt is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as HPLC purity, identity confirmation (IR, NMR), and water content. We adhere to relevant quality guidelines to support our global customer base in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.