Description

5-[3-(Aarboxymethoxy)Phenyl]-3-(4,5-Dimethyl-2-Thiazolyl)-2-(4-Sulfophenyl)-2H-Tetrazolium Inner Salt is a specialized tetrazolium salt derivative widely used as a critical reagent in biochemical and cell biology research. Its value lies in its role as a redox indicator, producing a measurable formazan dye upon reduction, which is essential for quantifying cell viability, proliferation, and cytotoxicity. This compound is primarily needed by research institutions, pharmaceutical R&D laboratories, and diagnostic kit manufacturers for applications in high-throughput screening and enzymatic assays.

Application

• Cell Viability and Proliferation Assays: Used as a key component in MTT and related assays to measure mitochondrial activity in live cells.
• Cytotoxicity Screening: Employed in pharmaceutical research to evaluate the toxic effects of drug candidates and chemical compounds on cell cultures.
• Biochemical Enzyme Assays: Serves as an electron acceptor in dehydrogenase and reductase enzyme activity tests.
• High-Throughput Screening (HTS): Integral to automated screening platforms in drug discovery for rapid assessment of compound libraries.
• Diagnostic Reagent Formulation: Used in the development of commercial diagnostic kits for clinical and research laboratories.
• Microbiology Research: Applied in studies of microbial cell growth and metabolic activity.

Basic Information

Product Name	5-[3-(Aarboxymethoxy)Phenyl]-3-(4,5-Dimethyl-2-Thiazolyl)-2-(4-Sulfophenyl)-2H-Tetrazolium Inner Salt
CAS No.	138169-43-4
Molecular Formula	C26H24N6O6S2
Molecular Weight	580.63 g/mol
Synonyms	MTS Tetrazolium Salt; 3-(4,5-Dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium; MTS Reagent; MTS; Inner Salt of MTS tetrazolium; CellTiter 96® AQueous One Solution Reagent (key component); Thiazolyl Blue Tetrazolium Bromide related compound; Water-soluble tetrazolium salt WST-1 (analog).
EINECS	Contact for details

Quality Control

Our 5-[3-(Aarboxymethoxy)Phenyl]-3-(4,5-Dimethyl-2-Thiazolyl)-2-(4-Sulfophenyl)-2H-Tetrazolium Inner Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. Due to its hygroscopic (moisture-sensitive) nature, the container should be kept in a desiccated environment after opening to prevent degradation. Avoid exposure to excessive heat.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility (in water)	Clear, yellow solution at 5 mg/mL
Absorbance (λ max)	Conforms (typically ~370-450 nm)
Biological Activity (Cell-based assay)	Conforms to performance standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.