Description

3-(5-((n-Biotinyl-6-Aminocaproyl)Amino)Pentyl)-1-(2-Deoxyerythropentofuranosyl)-1H-Pyrazolo(3,4-D)Pyrimidin-4-Amine-5'-Triphosphate is a sophisticated, biotin-labeled nucleotide analog designed for high-sensitivity detection and isolation applications. This compound is critical for researchers requiring precise, non-radioactive labeling of nucleic acids in complex biological systems. It is primarily utilized in advanced molecular biology and diagnostic development within the pharmaceutical, biotechnology, and life science research sectors.

Application

• Nucleic Acid Labeling & Detection: Acts as a biotinylated substrate for enzymatic incorporation into DNA or RNA probes for non-radioactive detection using streptavidin conjugates.
• Affinity Capture & Pull-Down Assays: Enables the specific isolation and purification of biotin-tagged nucleic acid complexes or associated proteins via streptavidin-coated beads.
• Next-Generation Sequencing (NGS): Used in specialized sequencing protocols that require biotin-labeled nucleotides for library preparation or target enrichment.
• Diagnostic Assay Development: Serves as a key component in the development of sensitive in-situ hybridization (ISH) and microarray-based diagnostic tests.
• Biochemical Research: Employed in studies of polymerase kinetics, enzyme mechanisms, and nucleic acid-protein interactions where a high-affinity tag is necessary.
• Probe Synthesis: For generating highly sensitive hybridization probes used in blotting techniques (Southern, Northern) and cytogenetic analyses.

Basic Information

Product Name	3-(5-((n-Biotinyl-6-Aminocaproyl)Amino)Pentyl)-1-(2-Deoxyerythropentofuranosyl)-1H-Pyrazolo(3,4-D)Pyrimidin-4-Amine-5'-Triphosphate
CAS No.	137823-47-3
Molecular Formula	C₃₈H₅₈N₁₁O₁₇P₃S
Molecular Weight	1102.93 g/mol
Synonyms	Biotin-11-dUTP; Biotin-11-2'-deoxyuridine-5'-triphosphate; 5-(Biotin-11-amidocaproylamidopentyl)-2'-deoxyuridine-5'-triphosphate; Biotinylated dUTP; Biotin-11-dUTP, triethylammonium salt; Biotin-11-2'-deoxyuridine triphosphate; 2'-Deoxy-5-[(N-(6-(biotinoylamino)hexanoyl)-5-aminopentyl)amino]uridine 5'-triphosphate; Biotin-11-UTP analog (DNA)
EINECS	Contact for details

Quality Control

Every batch of our Biotin-11-dUTP is manufactured and tested under stringent quality management systems to ensure the highest standards of purity and performance for critical research applications. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing key parameters such as HPLC purity, enzymatic incorporation efficiency, and the absence of contaminating nucleases. Our production adheres to cGMP guidelines where applicable, supporting use in regulated diagnostic and pharmaceutical development.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or lyophilized powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (Enzymatic)	≥ 90% incorporation efficiency
Water Content (KF)	≤ 5.0%
Solubility	Soluble in aqueous buffer (pH 7-8)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.