Description

7-Nitrobenz-2-Oxa-1,3-Diazole Desferrioxamine B is a fluorescently labeled derivative of the potent iron chelator Desferrioxamine B (DFO). This compound is engineered for advanced research applications, combining the high-affinity iron-binding properties of DFO with the sensitive detection capabilities of the NBD fluorophore. It is primarily utilized by researchers and scientists in the fields of biochemistry, molecular biology, and medicinal chemistry for studying iron metabolism, developing diagnostic probes, and investigating chelation therapy mechanisms.

Application

• Fluorescent Iron Sensing: Acts as a sensitive probe for the detection and quantification of Fe(III) ions in biological and environmental samples via fluorescence spectroscopy.
• Cellular Imaging: Used in fluorescence microscopy to visualize and track iron distribution and chelation within live or fixed cells.
• Biochemical Research: A critical tool for studying iron homeostasis, transport mechanisms, and the role of iron in various metabolic pathways.
• Diagnostic Development: Serves as a key component in the development of novel diagnostic assays for conditions related to iron overload or deficiency.
• Medicinal Chemistry: Employed in the design and evaluation of new therapeutic chelating agents for diseases like thalassemia and hemochromatosis.
• Material Science: Can be used to functionalize surfaces or nanoparticles for creating iron-sensitive biosensors or smart materials.

Basic Information

Product Name	7-Nitrobenz-2-Oxa-1,3-Diazole Desferrioxamine B
CAS No.	137756-28-6
Molecular Formula	C30H44N8O10
Molecular Weight	676.73 g/mol
Synonyms	NBD-Desferrioxamine B; NBD-DFO; 4-Nitro-2,1,3-benzoxadiazole Desferrioxamine B; 7-Nitrobenzo-2-oxa-1,3-diazole Desferrioxamine B; NBD-labeled Deferoxamine; Fluorescent Desferrioxamine B; NBD-Def; NBD-Conjugated Deferoxamine
EINECS	Contact for details

Quality Control

Our 7-Nitrobenz-2-Oxa-1,3-Diazole Desferrioxamine B is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and fluorescence spectroscopy for functional validation. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Custom synthesis and purification to meet specific project requirements are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep the container sealed in a dry, light-proof environment. Allow the product to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Fluorescence Properties	Ex/Em ~465/535 nm (solvent dependent)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.