Description

Human Adeno-Associated Virus Type-2 Rep Proteins are essential regulatory proteins critical for the site-specific integration and replication of the AAV-2 viral genome. These highly purified proteins are fundamental tools for advancing gene therapy research and viral vector production. They are primarily utilized by biopharmaceutical companies, CROs, and academic research institutions focused on developing and optimizing AAV-based gene delivery systems.

Application

• Gene Therapy Research: Essential component for studying AAV-2 replication mechanisms and developing recombinant AAV (rAAV) vectors.
• Viral Vector Production: Used in the manufacturing process of AAV-2-based gene therapy products to regulate viral DNA replication and packaging.
• Biochemical Assay Development: Serves as a key reagent in assays designed to screen for inhibitors or modulators of AAV replication.
• Cell Line Engineering: Employed in the creation of stable producer cell lines for scalable AAV vector production.
• Protein-Protein Interaction Studies: Used to investigate interactions between Rep proteins and host cell factors or viral ITR sequences.
• Reference Standard: Acts as a critical quality control standard for the characterization of AAV-based therapeutics.

Basic Information

Product Name	Human Adeno-Associated Virus Type-2 Rep Proteins
CAS No.	137750-19-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	AAV-2 Rep Proteins; Adeno-associated virus 2 Rep proteins; AAV2 Rep78; AAV2 Rep68; AAV2 Rep52; AAV2 Rep40; Recombinant AAV-2 Rep Proteins; AAV Replication Proteins; p5 Gene Products
EINECS	Contact for details

Quality Control

Our Human Adeno-Associated Virus Type-2 Rep Proteins are produced under stringent conditions to ensure high purity, identity, and biological activity suitable for research and bioprocessing applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity by SDS-PAGE, identity by Western Blot, and functional activity assays. We adhere to quality management principles aligned with ISO 9001 and cGMP guidelines where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). For long-term storage, aliquot and avoid repeated freeze-thaw cycles to maintain stability and activity.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Identification (Western Blot)	Positive for Rep78/Rep68 and Rep52/Rep40
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	As per COA
Endotoxin Level	< 1.0 EU/µg
Biological Activity (in vitro)	Functional in DNA binding or helicase assay
Solubility	Soluble in appropriate assay buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.