Description

o-Nitrophenyl-B-D-Glucuronide Potassium is a chromogenic substrate specifically designed for the detection and quantification of β-glucuronidase enzyme activity. This compound is critical for researchers and quality control laboratories requiring precise enzymatic assays. It is primarily utilized in the life sciences, pharmaceutical development, and diagnostic manufacturing sectors for applications ranging from high-throughput screening to microbiological testing.

Application

• Enzymatic Substrate for β-Glucuronidase: Used as a key reagent in biochemical assays to measure β-glucuronidase activity, which is a common reporter gene system.
• Microbiological Testing: Employed in culture media for the detection and identification of E. coli and other enteric bacteria that produce β-glucuronidase.
• Pharmaceutical Research & Development: Serves as a critical tool in drug discovery for screening enzyme inhibitors and studying drug metabolism pathways involving glucuronidation.
• Diagnostic Kit Manufacturing: An essential component in the formulation of clinical diagnostic kits for urinary tract infections and other bacterial assays.
• Cell Biology & Reporter Assays: Used in molecular and cellular biology to monitor gene expression and promoter activity when fused to the β-glucuronidase (GUS) gene.
• Food & Water Safety Analysis: Applied in methods for the rapid detection of fecal coliform contamination in water and food samples.

Basic Information

Product Name	o-Nitrophenyl-B-D-Glucuronide Potassium
CAS No.	137629-36-8
Molecular Formula	C12H13K2NO9
Molecular Weight	401.43 g/mol
Synonyms	o-Nitrophenyl β-D-Glucopyranosiduronic Acid Potassium Salt; ONPG Potassium Salt; ONP-Glucuronide Potassium Salt; Potassium (2-Nitrophenyl) β-D-Glucuronide; 2-Nitrophenyl-β-D-glucuronide Potassium Salt; β-Glucuronidase Substrate (ONPG); o-Nitrophenyl-β-D-glucuronide, Dipotassium Salt
EINECS	Contact for details

Quality Control

Every batch of o-Nitrophenyl-B-D-Glucuronide Potassium is manufactured and tested under a strict quality management system. We ensure compliance with high-purity standards suitable for research and diagnostic applications. Comprehensive testing includes identity confirmation, purity assay, and specific tests for residual solvents and moisture. A Certificate of Analysis (COA) detailing all specifications and test results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under inert atmosphere or with desiccant.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Clear, yellow solution in water
Specific Rotation	Contact for details
Heavy Metals	< 20 ppm
Residual Solvents (GC)	Meets ICH guidelines
Microbial Enumeration	< 100 CFU/g

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.