Description

Substance P, Biotin-Nte-Arg(3)- is a biotinylated derivative of the neuropeptide Substance P, specifically modified at the N-terminus with a biotin tag and an arginine tripeptide spacer. This engineered compound is crucial for advanced biochemical research, enabling highly specific detection, isolation, and analysis of Substance P and its receptor interactions. It is primarily utilized by researchers and developers in the fields of neuroscience, pharmacology, and molecular diagnostics for applications such as affinity purification, immunoassays, and receptor binding studies.

Application

• Affinity Chromatography & Purification: Used as a high-affinity ligand for isolating Substance P receptors or binding proteins from complex biological mixtures.
• Immunoassay Development: Serves as a critical capture or detection reagent in ELISA and other immunoassay formats for quantifying Substance P.
• Receptor Binding & Localization Studies: Employed in fluorescence microscopy (e.g., with streptavidin-fluorophore conjugates) or electron microscopy to visualize receptor distribution and internalization.
• Drug Discovery Screening: Acts as a tool compound in high-throughput screening (HTS) assays to identify and characterize novel agonists or antagonists for Substance P receptors (NK1R).
• Biomarker Research: Facilitates the study of Substance P's role in inflammatory processes, pain signaling, and neurological disorders.
• Biosensor Fabrication: Immobilized on sensor surfaces to create specific platforms for real-time analysis of ligand-receptor kinetics.

Basic Information

Product Name	Substance P, Biotin-Nte-Arg(3)-
CAS No.	137084-95-8
Molecular Formula	C90H146N30O21S
Molecular Weight	2036.4 g/mol
Synonyms	Biotin-Substance P; Biotinyl-Substance P; Biotin-Arg-Arg-Arg-Substance P; Biotin-Arg3-Substance P; Biotinylated Substance P (Arg3 spacer); Neurokinin P, Biotinyl-; H-Arg-Pro-Lys-Pro-Gln-Gln-Phe-Phe-Gly-Leu-Met-NH2 (Biotin-Arg3); SP-Biotin Conjugate
EINECS	Contact for details

Quality Control

Our Substance P, Biotin-Nte-Arg(3)- is manufactured under strict quality protocols suitable for research applications. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing specifications such as purity by HPLC, peptide content, and mass spectrometry (MS) confirmation are provided with every shipment to support your research integrity and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 70%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO, acetic acid solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.