Description

n-α-Cbz-Arg-Arg 7-Amido-4-Methylcoumarin Hydrochloride is a specialized fluorogenic peptide substrate designed for high-sensitivity protease detection. This compound is critical for biochemical research and diagnostic development, offering a cleavable amide bond that releases a highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety upon enzymatic hydrolysis. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for studying enzyme kinetics, screening protease inhibitors, and developing high-throughput assays.

Application

• Protease Activity Assays: A key fluorogenic substrate for the detection and quantification of trypsin-like serine proteases, including furin and related proprotein convertases.
• Drug Discovery Screening: Used in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic development.
• Biochemical Research: Essential for fundamental research into protease specificity, enzyme kinetics, and catalytic mechanisms in vitro.
• Diagnostic Kit Development: Employed in the formulation of sensitive diagnostic kits for detecting specific protease activities associated with diseases.
• Cell Biology Studies: Applied in cellular assays to monitor protease activation and activity within biological pathways.
• Quality Control in Biologics: Used to assess the activity and consistency of enzyme preparations in manufacturing processes.

Basic Information

Product Name	n-α-Cbz-Arg-Arg 7-Amido-4-Methylcoumarin Hydrochloride
CAS No.	136132-67-7
Molecular Formula	C32H44ClN9O7
Molecular Weight	702.20 g/mol
Synonyms	Z-Arg-Arg-AMC•HCl; Cbz-Arg-Arg-7-amido-4-methylcoumarin Hydrochloride; Benzyloxycarbonyl-Arg-Arg-AMC Hydrochloride; Furin Substrate; Proprotein Convertase Substrate; Boc-Arg-Val-Arg-Arg-MCA (related); Fluorogenic Peptide Substrate 136132-67-7; AMC-labeled Peptide Substrate
EINECS	Contact for details

Quality Control

Our n-α-Cbz-Arg-Arg 7-Amido-4-Methylcoumarin Hydrochloride is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and spectroscopic identification. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.