Description

Annexin V is a high-purity, recombinant protein widely recognized as a critical reagent for detecting phosphatidylserine (PS) exposure on the outer leaflet of the cell membrane, a hallmark of early-stage apoptosis. This specificity makes it an indispensable tool for life science research, drug discovery, and clinical diagnostics. It is essential for researchers and manufacturers in the fields of oncology, immunology, and toxicology who require reliable, consistent biomarkers for cell death assays.

Application

• Flow Cytometry (FACS): Primary application for the quantitative analysis of apoptotic cell populations in heterogeneous samples.
• Fluorescence Microscopy & Imaging: Used to visualize and localize apoptotic cells within tissues or cell cultures.
• Drug Screening & Development: Evaluates the efficacy and mechanisms of chemotherapeutic agents and other novel therapeutics by measuring induced apoptosis.
• Immunology Research: Studies apoptosis in immune cell regulation, autoimmune diseases, and vaccine development.
• Stem Cell Research: Monitors cell health and differentiation processes in stem cell cultures.
• Toxicology Studies: Assesses compound-induced cellular toxicity and safety profiles in preclinical research.
• Annexin V Binding Assay Kits: A key component in commercial assay kits for apoptosis detection, often conjugated to fluorophores like FITC or PE.

Basic Information

Product Name	Annexin V
CAS No.	136107-94-3
Molecular Formula	C214H347N63O63S2 (Approx., for human Annexin A5)
Molecular Weight	~35.9 kDa
Synonyms	Annexin A5; ANXA5; Anchorin CII; Calphobindin I; CBP-I; Endonexin II; Lipocortin V; Placental Anticoagulant Protein I (PAP-I); PP4; Thromboplastin Inhibitor; Vascular Anticoagulant-α (VAC-α)
EINECS	Contact for details

Quality Control

Our Annexin V is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot undergoes rigorous quality testing, including purity analysis by SDS-PAGE and HPLC, functional validation via calcium-dependent phospholipid binding assays, and endotoxin level testing. Certificates of Analysis (COA) detailing purity, concentration, and specific activity are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted solutions should be aliquoted and stored at -20°C to -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive).

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Purity (SDS-PAGE)	≥ 95%
Purity (HPLC)	≥ 98%
Identification (Mass Spec)	Conforms
Protein Content (BCA)	≥ 90%
Specific Activity (Binding)	Contact for details
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in aqueous buffers (e.g., PBS, HEPES) with calcium

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.