Description

Helix-Loop-Helix Protein 462 is a specialized recombinant protein belonging to the basic helix-loop-helix (bHLH) family of transcription factors. This protein is crucial for researchers investigating gene regulation, cellular differentiation, and developmental biology pathways. It is an essential reagent for biochemical assays, structural studies, and high-throughput screening in pharmaceutical discovery. Life science research institutions, biotechnology companies, and academic laboratories focused on molecular biology and drug target validation are the primary users of this high-purity protein.

Application

• Transcription Factor Research: Used in electrophoretic mobility shift assays (EMSAs) and chromatin immunoprecipitation (ChIP) to study DNA-binding activity and gene regulation mechanisms.
• Drug Discovery Screening: Serves as a target protein in high-throughput screening (HTS) campaigns to identify novel small-molecule modulators of bHLH protein function.
• Protein-Protein Interaction Studies: Employed in pull-down assays, co-immunoprecipitation (Co-IP), and surface plasmon resonance (SPR) to map interaction networks with partner proteins.
• Structural Biology: Provides material for X-ray crystallography, NMR spectroscopy, and cryo-electron microscopy to determine 3D structures and understand functional domains.
• Cell Signaling Pathway Analysis: Utilized in reporter gene assays and western blotting to dissect signaling cascades involving bHLH transcription factors in development and disease.
• Antibody Production & Validation: Acts as a high-quality antigen for generating and characterizing specific monoclonal or polyclonal antibodies.
• Biochemical Assay Development: Forms the core component in developing quantitative in vitro assays for enzymatic activity or inhibitor profiling.

Basic Information

Item	Details
Product Name	Helix-Loop-Helix Protein 462
CAS No.	135845-89-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	bHLH Protein 462; Transcription Factor bHLH462; Recombinant bHLH Protein; Basic Helix-Loop-Helix Protein 462; DNA-binding Protein 462; Class A bHLH Protein; Helix-Loop-Helix Transcriptional Regulator 462; HLH462
EINECS	Contact for details

Quality Control

Every batch of Helix-Loop-Helix Protein 462 is manufactured under stringent conditions and subjected to a comprehensive battery of analytical tests to ensure identity, purity, and functional activity. Our quality system aligns with best practices for research-grade biologics. A detailed Certificate of Analysis (COA) providing batch-specific data for purity (by SDS-PAGE and HPLC), endotoxin level, concentration, and functional activity is supplied with each shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C to -80°C. Avoid repeated freeze-thaw cycles. Aliquot as recommended upon receipt to maintain optimal activity.

Specification

Item	Specification
Appearance	Clear, colorless solution
Purity (SDS-PAGE)	≥ 95%
Purity (HPLC)	≥ 90%
Concentration	≥ 0.5 mg/mL
Buffer	Specified phosphate or Tris-based buffer
Endotoxin Level	< 1.0 EU/µg
Identification (Mass Spec)	Conforms
Biological Activity (EMSA)	Confirmed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.