Description

Sir1 Protein CAS NO 135844-02-9 is a high-purity recombinant protein derived from the Silent Information Regulator 1 (Sir1) family, known for its critical role in chromatin silencing and gene expression regulation. This product is essential for researchers and developers in the life sciences sector requiring a reliable and consistent reagent for advanced biochemical studies. It is primarily utilized in academic research, pharmaceutical R&D, and biotechnology applications focused on epigenetics, aging, and metabolic pathway analysis.

Application

• Biochemical Research: Used as a key reagent in chromatin immunoprecipitation (ChIP) assays and studies of histone deacetylase (HDAC) activity.
• Drug Discovery: Serves as a target protein in high-throughput screening (HTS) platforms for identifying novel epigenetic modulators and therapeutic agents.
• Enzymology Studies: Employed in kinetic assays to investigate NAD+-dependent deacetylase mechanisms and enzyme-substrate interactions.
• Cell Signaling Pathways: A vital tool for elucidating the role of sirtuins in cellular stress response, metabolism, and aging processes.
• Protein-Protein Interaction Analysis: Used in pull-down assays and surface plasmon resonance (SPR) to map molecular binding partners and complex formations.
• Diagnostic Development: Acts as a reference standard in the development of immunoassays and detection kits for sirtuin-related biomarkers.

Basic Information

Item	Details
Product Name	Sir1 Protein
CAS No.	135844-02-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	SIR1; Silent Information Regulator 1 Protein; NAD-dependent protein deacetylase sir1; Sirtuin 1 (SIRT1) homolog; Regulatory protein SIR1; Chromatin silencing protein Sir1; Yeast Sir1 protein homolog; Epigenetic regulatory protein; HDAC Class III protein; Gene silencing factor Sir1
EINECS	Contact for details

Quality Control

Our Sir1 Protein is manufactured under stringent quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment, SDS-PAGE for identity and homogeneity, and endotoxin level analysis to ensure suitability for sensitive biological applications. Certificates of Analysis (COA) detailing purity, activity, and specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Protein Content (BCA)	≥ 90%
Biological Activity	Contact for details
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.