Description

2-Chloro-4-Nitrophenyl-β-D-Cellobioside is a specialized chromogenic substrate designed for the sensitive and specific detection of cellulase enzyme activity. This compound matters because it provides a reliable, colorimetric readout for quantifying cellulolytic activity, which is critical for research and industrial process optimization. It is primarily needed by researchers in enzymology, biofuel production, and the pulp and paper industry for assessing cellulose degradation.

Application

• Enzyme Assays: As a chromogenic substrate for the quantitative determination of endo-1,4-β-glucanase (cellulase) activity in research and diagnostic kits.
• Biomass Conversion Research: Screening and characterization of novel cellulolytic enzymes for biofuel production from lignocellulosic materials.
• Industrial Process Monitoring: Used in quality control within industries like textiles, detergents, and animal feed to measure cellulase enzyme performance.
• Microbiological Studies: Detecting and studying cellulase-producing microorganisms in environmental and agricultural samples.
• Pharmaceutical Development: Potential use in assays for discovering cellulase inhibitors or activators for therapeutic applications.

Basic Information

Product Name	2-Chloro-4-Nitrophenyl-β-D-Cellobioside
CAS No.	135743-28-1
Molecular Formula	C18H24ClNO13
Molecular Weight	497.84 g/mol
Synonyms	4-Nitrophenyl 2-chloro-4-nitrophenyl β-D-cellobioside; CNP-Cellobioside; 2-Chloro-4-nitrophenyl β-cellobioside; β-4-Nitrophenyl 2-chloro-4-nitrophenyl-D-cellobioside; Cellulase Substrate; Chromogenic Cellulase Substrate; p-Nitrophenyl 2-chloro-4-nitrophenyl β-D-cellobioside
EINECS	Contact for details

Quality Control

Our 2-Chloro-4-Nitrophenyl-β-D-Cellobioside is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for sensitive enzymatic assays. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.