Description

4-Chloro-3-Indolyl β-D-Galactopyranoside is a chromogenic substrate widely used in molecular biology and diagnostic applications. Its core value lies in its enzymatic cleavage by β-galactosidase, producing an insoluble, intensely blue indigo dye that serves as a highly sensitive and visually distinct reporter. This compound is essential for researchers and manufacturers in biotechnology, pharmaceuticals, and in vitro diagnostics for applications such as gene expression assays, microbial detection, and histological staining.

Application

• Molecular Biology Research: As a key substrate in blue-white screening for recombinant clone selection in plasmid vectors containing the lacZ gene.
• Diagnostic Assays: Used in microbiology for the detection and identification of β-galactosidase-producing microorganisms, including E. coli and other coliforms.
• Enzyme Activity Studies: Employed in quantitative and qualitative assays to measure β-galactosidase activity in cell lysates and purified enzyme preparations.
• Histology & Cell Biology: Utilized in tissue staining and in situ hybridization techniques to localize gene expression at the cellular level.
• Pharmaceutical Screening: Applied in high-throughput screening (HTS) platforms to identify modulators of β-galactosidase activity for drug discovery.
• Food & Water Safety Testing: Incorporated into culture media and rapid test kits for the hygienic monitoring of food and water samples.

Basic Information

Product Name	4-Chloro-3-Indolyl β-D-Galactopyranoside
CAS No.	135313-63-2
Molecular Formula	C14H16ClNO6
Molecular Weight	329.73 g/mol
Synonyms	X-Gal; 5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside (common related compound, often searched together); 4-Chloro-3-indolyl-β-D-galactoside; β-Galactosidase Substrate; BCIG; Galactopyranoside, 4-chloro-3-indolyl β-D-; Chromogenic β-gal substrate; Bluo-Gal
EINECS	Contact for details

Quality Control

Our 4-Chloro-3-Indolyl β-D-Galactopyranoside is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and tests for residual solvents and moisture content. We provide a Certificate of Analysis (COA) with every shipment, detailing all specifications and test results to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, consider storage under desiccant or inert atmosphere. Keep away from strong acids and oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Heavy Metals	≤ 20 ppm
Biological Activity	Suitable for β-galactosidase detection

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.