Description

D-Luciferin, Ethyl Ester is a cell-permeable, synthetic analog of D-luciferin widely used as a substrate for firefly luciferase in bioluminescence assays. Its core value proposition lies in enabling highly sensitive, real-time monitoring of biological processes in live cells and animals for research and drug discovery applications. This compound is essential for researchers and developers in the pharmaceutical, biotechnology, and academic sectors who require reliable, high-purity reagents for advanced imaging and reporter gene studies.

Application

• Bioluminescence Imaging (BLI): Primary substrate for non-invasive, real-time monitoring of tumor growth, gene expression, and cell trafficking in live animal models.
• High-Throughput Screening (HTS): Used in cell-based assays to identify and characterize drug candidates, enzyme inhibitors, and receptor agonists/antagonists.
• Reporter Gene Assays: Enables the study of promoter activity, signal transduction pathways, and protein-protein interactions when coupled with luciferase genes.
• ATP Detection & Quantification: Serves as a critical component in sensitive assays for measuring cellular ATP levels, indicating cell viability and metabolic activity.
• Microbial Detection: Applied in rapid tests for microbial contamination by exploiting bacterial luciferase systems.
• In Vitro Diagnostic (IVD) Development: Used in the research and development of diagnostic kits utilizing bioluminescent signals.

Basic Information

Product Name	D-Luciferin, Ethyl Ester
CAS No.	135251-85-3
Molecular Formula	C14H12N2O3S2
Molecular Weight	320.39 g/mol
Synonyms	Ethyl D-Luciferin; D-(-)-2-(6-Hydroxy-2-benzothiazolyl)-4,5-dihydro-4-thiazolecarboxylic Acid Ethyl Ester; Ethyl (4S)-4,5-Dihydro-2-(6-hydroxy-1,3-benzothiazol-2-yl)-1,3-thiazole-4-carboxylate; D-Luciferin Ethyl Ester Potassium Salt (common salt form); (S)-2-(6-Hydroxybenzo[d]thiazol-2-yl)-4,5-dihydrothiazole-4-carboxylic Acid Ethyl Ester; Beetle Luciferin, Ethyl Ester; BioReagent for Luminescence; Firefly Luciferin Ethyl Ester
EINECS	Contact for details

Quality Control

Our D-Luciferin, Ethyl Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive biological assays. Each lot undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and functional testing in bioluminescence assays. Certificates of Analysis (COA) detailing purity, identity, and performance data are provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, light, or elevated temperatures will lead to degradation and reduced activity. For long-term storage, consider desiccant and under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Bioluminescence Activity	Passes functional assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.