Description

Biotin Amidite CAS NO 135137-87-0 is a high-purity phosphoramidite monomer specifically designed for the solid-phase synthesis of oligonucleotides. This compound is essential for introducing biotinylation sites into nucleic acid sequences, enabling downstream detection, purification, and immobilization applications. It is a critical raw material for biotechnology, pharmaceutical, and diagnostic companies engaged in the development of probes, aptamers, and therapeutic oligonucleotides.

Application

• Oligonucleotide Synthesis: Key building block for the automated solid-phase synthesis of biotin-labeled DNA and RNA strands.
• Diagnostic Probe Development: Used to create biotinylated probes for hybridization assays (e.g., ELISA, microarrays, in situ hybridization) where streptavidin-enzyme conjugates are used for signal amplification.
• Therapeutic Aptamer Production: Enables the site-specific incorporation of biotin into aptamers for targeted drug delivery or diagnostic applications.
• Next-Generation Sequencing (NGS): Employed in the preparation of biotinylated adapters or capture probes for library preparation and target enrichment.
• Affinity Purification: Facilitates the immobilization of oligonucleotides onto streptavidin-coated beads for purification or pull-down assays.
• Biosensor Fabrication: Used to attach nucleic acid capture sequences to streptavidin-functionalized surfaces in sensor devices.

Basic Information

Product Name	Biotin Amidite
CAS No.	135137-87-0
Molecular Formula	C39H51N6O7P
Molecular Weight	746.84 g/mol
Synonyms	5'-Dimethoxytrityl-N-biotinyl-6-aminohexyl-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Biotin Phosphoramidite; Biotin-dT Phosphoramidite (common trade name); Biotinylated Amidite; DMT-Biotin-dA-CE Phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-N6-(6-biotinamidohexyl)-2'-deoxyadenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; Biotin-TEG Amidite (related analog)
EINECS	Contact for details

Quality Control

Our Biotin Amidite is manufactured under strict quality control protocols to ensure consistency and reliability for critical oligonucleotide synthesis. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance metrics. We adhere to industry best practices and can provide material suitable for research and GMP-grade applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry environment. This product is hygroscopic (moisture-sensitive) and easily oxidized; allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.