Description

Wnt-3A Protein CAS NO 134944-05-1 is a highly purified, recombinant signaling protein crucial for regulating cell proliferation, fate determination, and stem cell maintenance. Its primary value lies in enabling advanced research into the Wnt/β-catenin signaling pathway, a fundamental mechanism in developmental biology and oncology. This protein is essential for biotechnology and pharmaceutical R&D teams focused on regenerative medicine, cancer research, and high-throughput drug screening applications.

Application

• Stem Cell Research & Culture: Used to maintain pluripotency and self-renewal of embryonic and adult stem cells in vitro.
• Cancer Biology Studies: Critical for investigating the role of Wnt signaling in tumor initiation, progression, and metastasis.
• Organoid Development: A key growth factor for the generation and long-term culture of complex 3D organoid models from various tissues.
• Drug Discovery & Screening: Employed in cell-based assays to identify and validate novel therapeutic compounds that modulate the Wnt pathway.
• Developmental Biology: Used to study cell fate specification, tissue patterning, and embryogenesis in model systems.
• Regenerative Medicine: Applied in protocols for directed differentiation of stem cells into specific lineages for tissue engineering.
• Biochemical Assays: Serves as a critical reagent in ELISA, Western blot, and other protein interaction studies to analyze pathway activity.

Basic Information

Product Name	Wnt-3A Protein
CAS No.	134944-05-1
Molecular Formula	Contact for details
Molecular Weight	~39-42 kDa (glycosylated form)
Synonyms	Wnt-3A; Wnt3A Protein; Recombinant Wnt-3A; Wingless-type MMTV integration site family, member 3A; Protein Wnt-3a; Wnt Family Member 3A; rWnt3A
EINECS	Contact for details

Quality Control

Our Wnt-3A Protein is produced under stringent conditions and undergoes comprehensive analytical testing to ensure high biological activity, purity, and batch-to-batch consistency. Quality is verified through methods including SDS-PAGE, HPLC, and functional bioassays. A detailed Certificate of Analysis (COA) documenting purity, endotoxin level, sterility, and specific activity is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, keep frozen at -20°C to -80°C in aliquots to avoid repeated freeze-thaw cycles. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature before opening to prevent condensation. Store under conditions that minimize exposure to moisture.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Purity (SDS-PAGE)	> 95%
Endotoxin Level	< 1.0 EU/μg
Biological Activity	Determined by cell-based assay (e.g., TOPFlash reporter assay)
Sterility	Sterile-filtered (0.2 μm)
Solubility	Soluble in PBS or cell culture medium
Identification (HPLC/MS)	Conforms to standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.