Description

Ure2 Protein CAS NO 134773-68-5 is a high-purity recombinant protein derived from yeast, specifically known for its role as a transcriptional regulator in nitrogen metabolism. This product is essential for researchers and developers in the life sciences sector requiring a reliable and well-characterized protein for functional studies and assay development. It is particularly valuable for academic, pharmaceutical, and biotechnology laboratories focused on molecular biology, protein-protein interactions, and metabolic pathway analysis.

Application

• Biochemical Research: Used as a key reagent in studies of nitrogen catabolite repression (NCR) and gene regulation in Saccharomyces cerevisiae.
• Protein Interaction Studies: Serves as a target protein in yeast two-hybrid screens, pull-down assays, and co-immunoprecipitation experiments to identify binding partners.
• Enzyme Activity Assays: Employed in in vitro assays to investigate its functional properties and regulatory mechanisms.
• Diagnostic Development: Potential use as an antigen or standard in the development of immunological assays and diagnostic kits.
• Educational Purposes: Supplied to academic institutions for teaching advanced concepts in molecular genetics and protein biochemistry.
• Drug Discovery Screening: Utilized in high-throughput screening platforms to identify molecules that modulate Ure2p function, relevant for metabolic disorder research.

Basic Information

Product Name	Ure2 Protein
CAS No.	134773-68-5
Molecular Formula	Contact for details
Molecular Weight	~40 kDa (monomer, contact for exact lot-specific data)
Synonyms	Ure2p; Nitrogen catabolite repression regulatory protein URE2; URE2 transcriptional regulator; Yeast Ure2 protein; GLN3 regulator URE2; Ure2p prion domain protein; Recombinant S. cerevisiae Ure2 protein
EINECS	Contact for details

Quality Control

Our Ure2 Protein is produced under stringent conditions to ensure high batch-to-batch consistency and biological activity. Each lot undergoes comprehensive analytical testing, including SDS-PAGE for purity assessment, Western Blot for identity confirmation, and functional activity assays. A comprehensive Certificate of Analysis (COA) detailing purity, concentration, buffer composition, and storage conditions is provided with every shipment to guarantee it meets the rigorous standards required for research and development.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed properly after each use. Store away from strong acids and organic solvents.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Identification (Western Blot)	Positive for Ure2p
Protein Concentration	≥ 0.5 mg/mL (lot-specific)
Buffer Composition	Tris-HCl, NaCl, Glycerol, DTT (detailed info on COA)
Endotoxin Level	< 1.0 EU/µg (for applicable grades)
Biological Activity	Confirmed by functional assay (e.g., binding assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.