Description

Prp22 Protein CAS NO 134711-02-7 is a highly purified recombinant protein essential for pre-mRNA splicing research and development. This product is critical for studying the intricate mechanisms of spliceosome assembly and function, providing researchers with a reliable and consistent reagent. It is primarily utilized by biotechnology firms, academic research institutions, and pharmaceutical companies engaged in molecular biology, genetic research, and the development of novel therapeutic strategies targeting RNA processing.

Application

• Spliceosome Research: A key component for in vitro studies of spliceosome assembly, activation, and catalytic steps.
• Biochemical Assay Development: Used as a standard or reagent in developing high-throughput screening assays for splicing modulators.
• Protein-Protein Interaction Studies: Employed to investigate interactions with other splicing factors like Prp8, Brr2, and Snu114 within the U5 snRNP complex.
• Enzymology Studies: Essential for analyzing the ATP-dependent RNA helicase activity crucial for spliceosome remodeling.
• Antibody Production & Validation: Serves as a high-purity antigen for generating and validating specific antibodies used in Western blotting and immunoprecipitation.
• Diagnostic Tool Development: Potential use in creating research tools for understanding diseases linked to splicing defects, such as certain cancers and genetic disorders.

Basic Information

Item	Detail
Product Name	Prp22 Protein
CAS No.	134711-02-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Pre-mRNA-processing-splicing factor ATP-dependent RNA helicase PRP22; DEAH-box protein 8; DHX8; KIAA0831; Prp22p; hPrp22; Splicing factor, ATP-dependent RNA helicase PRP22; DEAD/H-box RNA helicase PRP22
EINECS	Contact for details

Quality Control

Our Prp22 Protein is produced under stringent conditions to ensure high purity and consistent biological activity. Each batch undergoes comprehensive analytical testing, including SDS-PAGE, Western Blot, and functional activity assays. Certificates of Analysis (COA) detailing purity, concentration, and specific activity are provided to guarantee the product meets the exacting standards required for critical research applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after each use.

Specification

Item	Specification
Appearance	Clear or slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Concentration	≥ 0.5 mg/mL
Buffer	Specified buffer solution (e.g., Tris, HEPES) with stabilizer
Identification (Western Blot)	Positive
Biological Activity	Confirmed by in vitro splicing or ATPase/helicase assay
Endotoxin Level	< 1.0 EU/µg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.