Description

5-Bromo-4-Chloro-3-Indoxyl Nonanoate is a chromogenic enzyme substrate widely used in biochemical and diagnostic applications. This compound is valued for its role in producing an insoluble, colored precipitate upon enzymatic cleavage, enabling precise detection and localization of specific enzymatic activity. It is an essential reagent for researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors who require reliable, high-purity substrates for histochemistry, microbiology, and molecular biology.

Application

• Primary substrate for the detection of esterase and lipase activity in histochemical staining and blotting techniques.
• Used in the formulation of culture media and diagnostic kits for microbiological identification and research.
• Critical reagent in molecular biology for visualizing gene expression and reporter gene assays.
• Employed in the development of biochemical sensors and assays for pharmaceutical and environmental testing.
• Essential for neuroscience and cell biology research to study enzymatic processes in tissues and cells.
• Used in industrial enzymology for screening and characterizing novel enzymes.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl Nonanoate
CAS No.	133950-77-3
Molecular Formula	C₁₇H₂₁BrClNO₃
Molecular Weight	402.71 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl nonanoate; BCIP Nonanoate; X-Nonanoate; 3-Indoxyl Nonanoate, 5-Bromo-4-chloro derivative; Nonanoic acid 5-bromo-4-chloro-1H-indol-3-yl ester; Chromogenic esterase substrate; Indoxyl ester substrate
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indoxyl Nonanoate is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are provided to guarantee the material meets the stringent requirements for diagnostic and research-grade applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. For long-term storage, keep the container under an inert atmosphere and avoid repeated freeze-thaw cycles to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%
Solubility	Soluble in DMSO, DMF; slightly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.