Description

Naphthofluorescein Di-(β-D-Galactopyranoside) CAS NO 133551-98-1 is a specialized fluorogenic substrate designed for high-sensitivity enzymatic detection. This compound is critical for researchers and diagnostic developers requiring precise and reliable measurement of β-galactosidase activity. It is primarily utilized in life science research, clinical diagnostics, and pharmaceutical development for applications such as reporter gene assays and cell-based screening.

Application

• Reporter Gene Assays: A key substrate for detecting β-galactosidase expression in molecular biology and genetic engineering studies.
• Enzyme Activity Measurement: Used in biochemical assays to quantify β-galactosidase activity with high sensitivity due to its fluorogenic properties.
• Cell-Based Screening: Enables the identification and analysis of gene expression in live cells for drug discovery and functional genomics.
• Diagnostic Kit Development: Serves as a core component in the development of diagnostic tests for bacterial detection (e.g., E. coli) and certain genetic disorders.
• High-Throughput Screening (HTS): Ideal for automated screening platforms in pharmaceutical research to identify enzyme inhibitors or activators.
• Histochemistry & Microscopy: Used in tissue staining and imaging techniques to localize β-galactosidase activity at the cellular level.

Basic Information

Product Name	Naphthofluorescein Di-(β-D-Galactopyranoside)
CAS No.	133551-98-1
Molecular Formula	C40H40O17
Molecular Weight	792.74 g/mol
Synonyms	Naphthofluorescein di-β-D-galactopyranoside; β-Galactosidase Substrate Naphthofluorescein Di-β-D-Galactoside; Naphthofluorescein Di-β-D-Galactoside; NFDG; 5(6)-Carboxy-2',7'-dichlorofluorescein Di-(β-D-Galactopyranoside); 5(6)-Carboxy-2',7'-dichlorofluorescein di-β-D-galactoside; CDCFDG; 6-Carboxy-2',7'-dichlorofluorescein di-β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our Naphthofluorescein Di-(β-D-Galactopyranoside) is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to meet the exacting standards of research and diagnostic use. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, light, or elevated temperatures will compromise stability and performance. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, DMF
Fluorogenic Activity	Conforms to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.